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Virginia Commonwealth/16 July 2009
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[[User:Trentay|Trentay]] 14:31, 16 July 2009 (UTC) | [[User:Trentay|Trentay]] 14:31, 16 July 2009 (UTC) | ||
| - | + | ''Craig and Clay'' | |
* No growth was seen on either the kanamycin or chloramphenicol plates. | * No growth was seen on either the kanamycin or chloramphenicol plates. | ||
* The sequence for the LIM1 gene was verified to be optimized for E. coli. | * The sequence for the LIM1 gene was verified to be optimized for E. coli. | ||
Revision as of 14:15, 24 July 2009
Contents |
Thursday 16 July 2009
Results
- Results of Retransformation with pSB4C5 backbone in NEB 10 beta two days after transformation
| Promoter | Reporter | Resistance/Plate | Growth Observation | |
|---|---|---|---|---|
| J23101 | E0240 | Cm | no growth | |
| J23101 | J06702 | Cm | 21 colonies | |
| J23102 | E0240 | Cm | 3 colonies | |
| J23102 | J06702 | Cm | 8 colonies | |
| J23103 | E0240 | Cm | 2 colonies | |
| J23103 | J06702 | Cm | 23 colonies | |
| J23104 | E0240 | Cm | 9 colonies | |
| J23104 | J06702 | Cm | 12 colonies | |
| J23105 | E0240 | Cm | no growth | |
| J23105 | J06702 | Cm | 14 colonies | |
| J23107 | E0240 | Cm | 3 colonies | |
| J23107 | J06702 | Cm | 3 colonies |
- No growth was seen in 5mL culture taken from NEB 10 beta cell freezer stock
- Vial may have thawed out too much after cells were taken out for the electrocompetency procedure
Trentay 14:31, 16 July 2009 (UTC)
Craig and Clay
- No growth was seen on either the kanamycin or chloramphenicol plates.
- The sequence for the LIM1 gene was verified to be optimized for E. coli.
Tasks
Maria and Afton
- Digest: E0240,J06702, pSB4C5, 110, 113, 116, 0011, 0040
- Ligate promoters with backbone pSB4C5 and reporters E0240, J06702
- Run an electrophoresis test on promoters and reporter Eo240
- Pick colonies of promoters from transformation 100-107 (excluding 106)
- Transformation of NEB 10 beta to make sure new stock is electrocompetent
Trentay 20:41, 16 July 2009 (UTC)
Craig and Clay
- The registry will be contacted to verify the correct resistence for the limonene synthesis related parts. New DNA will also be requested.
Wetlab
Maria and Afton
- Digest: E0240,J06702, pSB4C5, 110, 113, 116, 0011, 0040
- Ligate promoters with backbone pSB4C5 and reporters E0240, J06702
- Run an electrophoresis test on promoters and reporter Eo240
Trentay 20:41, 16 July 2009 (UTC)
