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- | <div id="header">{{Template:Team:Virginia_Commonwealth/Templates/Header}}</div>
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- | <table>
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- | <html>
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- | <style type="text/css">
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- | table.calendar { margin: 0; padding: 2px; }
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- | table.calendar td { margin: 0; padding: 1px; vertical-align: top; }
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- | table.month .heading td { padding:0px; background-color: black; color: gold; text-align:center; font-size:100%; font-weight:bold; }
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- | table.month .dow td { color:#000000; text-align:center; font-size:100%; }
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- | table.month td.today { background-color:#cd0000; }
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- | table.month td {
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- | border: none;
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- | margin: 0;
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- | padding: 0pt 0.5pt;
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- | font-weight: bold;
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- | font-size: 10pt;
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- | text-align: right;
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- | background-color: white;
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- | }
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- | #bodyContent table.month a { background:none; padding:0 }
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- | .day-active { color:#cd0000 }
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- | .day-empty { color:#000000 }
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- | </style>
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- | </html>
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- |
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- |
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- | {|style="font color="#ffffff"; "background-color:"#cd0000"; cellpadding="0" cellspacing="4" border="4" bordercolor="#000"; border-spacing:0px; text-align:center" width="250px"
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- | </table>
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- |
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- | {| align="center"
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- | |-valign="top"
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- | |align="center" width="150pt"|{{#calendar: title=Virginia_Commonwealth |year=2009 | month=05}}
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- | |align="center" width="150pt"|{{#calendar: title=Virginia_Commonwealth |year=2009 | month=06}}
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- | |align="center" width="150pt"|{{#calendar: title=Virginia_Commonwealth |year=2009 | month=07}}
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- | |align="center" width="150pt"|{{#calendar: title=Virginia_Commonwealth |year=2009 | month=08}}
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- | |align="center" width="150pt"|{{#calendar: title=Virginia_Commonwealth |year=2009 | month=09}}
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- | |align="center" width="150pt"|{{#calendar: title=Virginia_Commonwealth |year=2009 | month=10}}
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- | |}
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- | ==Monday 20 July 2009==
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- | ===Results===
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- | * Results of transformation with pSB4C5 backbone in NEB 10 beta three days after transformation
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- | ** Control (+) on LB NEB 10 beta (not shocked) had Lawn growth
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- | ** Control (-) on Cm NEB 10 beta (not shocked) had No growth
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- |
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- | {| cellpadding="10" align="center" border="0.9" padding="5"
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- | |+
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- | |-
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- | ! Promoter!! Reporter !! Resistance/Plate !! Growth Observation !!
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- | |-
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- | ! J23110
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- | | E0240 || Cm || 62 colonies ||
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- | |-
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- | ! J23110
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- | | J06702 || Cm || 56 colonies ||
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- | |-
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- | ! J23113
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- | | E0240 || Cm || 75 colonies ||
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- | |-
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- | ! J23113
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- | | J06702 || Cm || 33 colonies ||
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- | |-
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- | ! J23116
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- | | E0240 || Cm || 41 colonies ||
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- | |-
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- | ! J23116
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- | | J06702 || Cm || 65 colonies ||
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- | |-
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- | ! R0011
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- | | E0240 || Cm || hundreds of colonies ||
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- | |-
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- | ! R0011
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- | | J06702 || Cm || 5 colonies ||
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- | |-
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- | ! R0040
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- | | E0240 || Cm || 20 colonies ||
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- | |-
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- | ! R0040
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- | | J06702 || Cm || 12 colonies ||
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- | |-
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- | ! J23105
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- | | E0240 || Cm || 26 colonies ||
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- | |-
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- | ! J23101
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- | | E0240 || Cm || 34 colonies ||
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- | |}
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- | [[User:Trentay|Trentay]] 14:17, 20 July 2009 (UTC)
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- |
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- | ''Craig and Clay''
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- | * The registry did confirm that the correct resistance for the limonene synthesis related parts is ampicillin. New parts will also be sent.
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- | ----
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- |
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- | ===Tasks===
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- | ''Maria and Afton''
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- | * Pick colonies for overnight culture from transformations
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- | * Ligate J23106 and J23101 with backbone pSB4C5 and RET E0240
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- | * Transform liagated parts into NEB 10 beta
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- | [[User:Trentay|Trentay]] 18:40, 20 July 2009 (UTC)
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- | ----
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- |
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- | ====Wetlab====
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- | *1
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- | *2
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