"
EPF-Lausanne/7 July 2009
From 2009.igem.org
(Difference between revisions)
(→People in the lab) |
|||
| Line 54: | Line 54: | ||
<form action="input_button.htm"> | <form action="input_button.htm"> | ||
<p> | <p> | ||
| - | <input type="button" name="lien" value=" | + | <input type="button" name="lien" value="8 July 2009" |
| - | onClick="self.location.href=' | + | onClick="self.location.href='https://2009.igem.org/EPF-Lausanne/8_July_2009'"> |
</p> | </p> | ||
</form> | </form> | ||
| - | |||
</body> | </body> | ||
</html> | </html> | ||
Revision as of 07:53, 28 July 2009
Wet Lab
We have to grow the 3 strains generously sent by Tom Beatty
The three strains are :
- R.Palustris CEA001 (wild type) ; should be grown on LB medium only
- R.Palustris BPHP1+ ; should be grown on LB with gentamycin (100 micrograms/ml)
- E.Coli DH10B (pBPH/hmu0) ; should be grown on LB with gentamycin (20 micorgrams/ml)
The transformed LOVTAP and TrpR worked well (N.B. the plasmid of TrpR is pUC19 so the antibiotic resistance is Amp -> see below)
We did the glycerol stock, located in the -80 fridge, first floor of the iGEM compartement.
Then, a miniprep was done with both cultures. A LOVTAP plasmid aliquot was done, a TrpR plasmid aliquot was done, located in the -20 fridge, 2nd floor.
Cloning Strategy
To design plasmids : software Vector NTI
People in the lab
- Tu, Heidi, Rafael, Basile, Nath
