"
EPF-Lausanne/7 July 2009
From 2009.igem.org
(Difference between revisions)
(→People in the lab) |
(→People in the lab) |
||
| Line 65: | Line 65: | ||
<div> | <div> | ||
<button name="cliquemoi" type="button" | <button name="cliquemoi" type="button" | ||
| - | value="SELFHTML" onClick="self.location.href=' | + | value="SELFHTML" onClick="self.location.href='https://2009.igem.org/EPF-Lausanne/8_July_2009'"> |
| - | <p><img src=" | + | <p><img src="https://static.igem.org/mediawiki/2009/thumb/5/5e/Fleche_droite.png/70px-Fleche_droite.png" width="106" height="109" border="0" alt="SELFHTML"><br> |
| - | <b> | + | <b>8 July 2009</b></p> |
</button> | </button> | ||
</div> | </div> | ||
Revision as of 07:59, 28 July 2009
Wet Lab
We have to grow the 3 strains generously sent by Tom Beatty
The three strains are :
- R.Palustris CEA001 (wild type) ; should be grown on LB medium only
- R.Palustris BPHP1+ ; should be grown on LB with gentamycin (100 micrograms/ml)
- E.Coli DH10B (pBPH/hmu0) ; should be grown on LB with gentamycin (20 micorgrams/ml)
The transformed LOVTAP and TrpR worked well (N.B. the plasmid of TrpR is pUC19 so the antibiotic resistance is Amp -> see below)
We did the glycerol stock, located in the -80 fridge, first floor of the iGEM compartement.
Then, a miniprep was done with both cultures. A LOVTAP plasmid aliquot was done, a TrpR plasmid aliquot was done, located in the -20 fridge, 2nd floor.
Cloning Strategy
To design plasmids : software Vector NTI
People in the lab
- Tu, Heidi, Rafael, Basile, Nath
