Team:EPF-Lausanne/Notebook/Wet Lab

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(New page: =Wet Lab= ==06.07.09== LOVTAP plasmid AND TrpR plasmid were transformed in competent E. Coli following received protocol, and grown overnight (see Lab book for more details). <br>One probl...)
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=Wet Lab=
 
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==06.07.09==
 
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LOVTAP plasmid AND TrpR plasmid were transformed in competent E. Coli following received protocol, and grown overnight (see Lab book for more details).
 
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<br>One problem: we actually don't know TrpR plasmid resistance, so we tried with three resistances available in the lab: Amp., Kana. and Chl.
 
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LOVTAP is in a plasmid called pCal-n (see picture below):
 
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[[Image: pCAL-n.jpg|500px|thumb|center|pCal-n plasmid]]
 
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<br>Some comments on the plasmid:
 
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<br>-CBP is a small peptide with which we could purify LOVTAP protein
 
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<br>-Thrombin target is a nucleotidic sequence that can be recognized by thrombin a peptidase. This peptidase will cut CBP once LOVTAP is purified
 

Latest revision as of 08:05, 28 July 2009

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