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| - | {{EPF-Lausanne09}}
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| - | <div CLASS="epfltrick">__TOC__
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| - | </div><div CLASS="epfl09">
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| - | =Wet Lab=
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| - | ==06.07.09==
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| - | LOVTAP plasmid AND TrpR plasmid were transformed in competent E. Coli following received protocol, and grown overnight (see Lab book for more details).
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| - | <br>One problem: we actually don't know TrpR plasmid resistance, so we tried with three resistances available in the lab: Amp., Kana. and Chl.
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| - | LOVTAP is in a plasmid called pCal-n (see picture below):
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| - | [[Image: pCAL-n.jpg|500px|thumb|center|pCal-n plasmid]]
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| - | <br>Some comments on the plasmid:
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| - | <br>-CBP is a small peptide with which we could purify LOVTAP protein
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| - | <br>-Thrombin target is a nucleotidic sequence that can be recognized by thrombin a peptidase. This peptidase will cut CBP once LOVTAP is purified
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| - | </div><div CLASS="epfl09bouchon"></div>
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Latest revision as of 08:05, 28 July 2009
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