From 2009.igem.org
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| - | {{EPF-Lausanne09}}
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| - | <div CLASS="epfltrick">__TOC__
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| - | </div><div CLASS="epfl09">
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| - | =Cloning strategy=
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| - | ==July==
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| - |
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| - | ===06.07.09===
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| - | Four forward primers were designed to amplify:
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| - | <br> 1.Promoter T7, RBS, CBP and LOVTAP:
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| - | :gtttcttcgaattcgcggccgcttctagagtaatacgactcactataggggaattgtg
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| - | 2.RBS, CBP and LOVTAP:
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| - | :gtttcttcgaattcgcggccgcttctagagtgtttaactttaagaaggag
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| - | 3.CBP and LOVTAP:
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| - | :gtttcttcgaattcgcggccgcttctagatgaagcgacgatggaaaaagaatttcatag
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| - | 4.LOVTAP:
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| - | :gtttcttcgaattcgcggccgcttctagatgctactacacttgaacgtattgagaagaac
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| - | One reverse primer were designed:
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| - | :gtttcttcctgcagcggccgctactagtatcaatcgcttttcagcaacacctcttc
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| - | The '''recipient IGEM part''' have been chosen: [http://partsregistry.org/partsdb/get_part.cgi?part=BBa_B0010 BBa_B0010], well 13D in the received kit plate 1
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| - |
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| - | ===07.07.09===
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| - | To design plasmids : software Vector NTI
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| - |
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| - | ===08.07.09===
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| - |
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| - | ===09.07.09===
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| - |
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| - | ===10.07.09===
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| - |
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| - | ===13.07.09===
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| - | Restriction enzymes on Biolabs website [http://www.neb.com/nebecomm/products/category1.asp?#2 Biolabs website]
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| - |
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| - | ===14.07.09===
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| - |
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| - | ===15.07.09===
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| - |
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| - | ===16.07.09===
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| - |
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| - | ===17.07.09===
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| - |
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| - | ==August==
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| - | </div><div CLASS="epfl09bouchon"></div>
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Latest revision as of 08:05, 28 July 2009
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