EPF-Lausanne/7 July 2009

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(New page: ===Wet Lab=== We have to grow the 3 strains generously sent by [mailto:j.beatty@ubc.ca Tom Beatty] The three strains are : :*''R.Palustris'' CEA001 (wild type) ; should be grown on LB med...)
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==Wet Lab==
We have to grow the 3 strains generously sent by [mailto:j.beatty@ubc.ca Tom Beatty]
We have to grow the 3 strains generously sent by [mailto:j.beatty@ubc.ca Tom Beatty]
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Then, a miniprep was done with both cultures.  
Then, a miniprep was done with both cultures.  
A LOVTAP plasmid aliquot was done, a TrpR plasmid aliquot was done, located in the -20 fridge, 2nd floor.
A LOVTAP plasmid aliquot was done, a TrpR plasmid aliquot was done, located in the -20 fridge, 2nd floor.
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==Cloning Strategy==
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To design plasmids : software Vector NTI
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==People in the lab==
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:Tu, Heidi, Rafael, Basile, Nath
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Latest revision as of 08:49, 28 July 2009



Contents


7 July 2009



Wet Lab

We have to grow the 3 strains generously sent by Tom Beatty

The three strains are :

  • R.Palustris CEA001 (wild type) ; should be grown on LB medium only
  • R.Palustris BPHP1+ ; should be grown on LB with gentamycin (100 micrograms/ml)
  • E.Coli DH10B (pBPH/hmu0) ; should be grown on LB with gentamycin (20 micorgrams/ml)


The transformed LOVTAP and TrpR worked well (N.B. the plasmid of TrpR is pUC19 so the antibiotic resistance is Amp -> see below)


pUC19 plasmid


We did the glycerol stock, located in the -80 fridge, first floor of the iGEM compartement.

Then, a miniprep was done with both cultures. A LOVTAP plasmid aliquot was done, a TrpR plasmid aliquot was done, located in the -20 fridge, 2nd floor.


Cloning Strategy

To design plasmids : software Vector NTI


People in the lab

Tu, Heidi, Rafael, Basile, Nath



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