Team:MoWestern Davidson/tRNA
From 2009.igem.org
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<blockquote> The underlined portion of the oligos are the anti codon loop and its complementary bases on the opposite strand. The different colors represent the different oligo nucleotides. The top strand has 5' and 3' overhangs which are the sticky ends for EcoRI and PstI respectivly. Our team annealed the oligos together and then cloned them into a pSB1A2 vector.</blockquote> | <blockquote> The underlined portion of the oligos are the anti codon loop and its complementary bases on the opposite strand. The different colors represent the different oligo nucleotides. The top strand has 5' and 3' overhangs which are the sticky ends for EcoRI and PstI respectivly. Our team annealed the oligos together and then cloned them into a pSB1A2 vector.</blockquote> | ||
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Revision as of 15:35, 28 July 2009
The underlined portion of the oligos are the anti codon loop and its complementary bases on the opposite strand. The different colors represent the different oligo nucleotides. The top strand has 5' and 3' overhangs which are the sticky ends for EcoRI and PstI respectivly. Our team annealed the oligos together and then cloned them into a pSB1A2 vector.