From 2009.igem.org
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- | AMPLIFICATION OF ''LUXCDABE'' USING TAQ POLYMERASE | + | AMPLIFICATION OF <i>LUXCDABE</i> USING TAQ POLYMERASE |
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Revision as of 16:17, 28 July 2009
University of Calgary
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CAROL
AMPLIFICATION OF LUXCDABE USING TAQ POLYMERASE
THE PURPOSE OF THIS EXPERIMENT WAS TO AMPLIFY LUXCDABE WITH TAQ POLYMERASE. GENE SPECIFIC LUXCDABE FORWARD AND REVERSE PRIMERS WERE USED. CONDITIONS USED: DENATURE (94 DEGREES CELSIUS, 3 MINUTES), 30 CYCLES (DENATURE (94 DEGREES CELSIUS, 45 SECONDS), ANNEAL (52 DEGREES CELSIUS, 45 SECONDS), EXTEND (72 DEGREES CELSIUS, 6 MINUTES)), FINAL EXTENSION (72 DEGREES CELSIUS, 10 MINUTES), AND HOLD AT 4 DEGREES CELSIUS OVERNIGHT.
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CHINMOYEE
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EMILY
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FAHD
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IMAN
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JAMIE
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JEREMY
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KATIE
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KEVIN
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MANDY
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PATRICK
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PRIMA
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STEFAN
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VICKI
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