Uppsala-Sweden/27 July 2009

From 2009.igem.org

(Difference between revisions)
(New page: 2009-07-27 11:15,OD measurement of Butanol at 750nm)
 
(3 intermediate revisions not shown)
Line 1: Line 1:
-
2009-07-27 11:15,OD measurement of Butanol at 750nm
+
2009-07-27 11:15,OD measurement of Butanol at 750nm,
 +
 
 +
pirA PCR was conducted, using
 +
1. Primer:pirA forward
 +
      pirA reverse
 +
      pirB forward
 +
  at three different template concentration,
 +
 
 +
2. Primer:pirA reverse
 +
          pirB forward
 +
at three different template concentration,
 +
Control pirB
 +
 
 +
PCR PROGRAM
 +
denaturing temperature 98
 +
denaturing time: 30s
 +
cycle: 5
 +
denaturing temperature 98
 +
denaturing time: 10s
 +
anealing temperature: 63, time 30s
 +
extension time:32s 
 +
extension temperature 72
 +
cycle: 30
 +
denaturing temperature 98
 +
denaturing time: 10s
 +
annealing temperature: 72,time 30s
 +
extension time:32s 
 +
extension temperature 72
 +
hold 5 min
 +
 
 +
 
 +
 
 +
     
 +
-- Emily

Latest revision as of 14:10, 30 July 2009

2009-07-27 11:15,OD measurement of Butanol at 750nm,

pirA PCR was conducted, using 1. Primer:pirA forward 

      pirA reverse 
      pirB forward
  at three different template concentration,

2. Primer:pirA reverse 

         pirB forward
at three different template concentration,

Control pirB

PCR PROGRAM denaturing temperature 98 denaturing time: 30s cycle: 5 denaturing temperature 98 denaturing time: 10s anealing temperature: 63, time 30s extension time:32s extension temperature 72 cycle: 30 denaturing temperature 98 denaturing time: 10s annealing temperature: 72,time 30s extension time:32s extension temperature 72 hold 5 min



-- Emily

Retrieved from "http://2009.igem.org/Uppsala-Sweden/27_July_2009"