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Minnesota/9 July 2009
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| + | '''Patrick'''<br> | ||
| + | Today I received and analyzed the three models created yesterday, smad_ttn_gfp_u, smad_ttn_gfp_d, smad_ttn_gfp_d2. Below are the first two models, where the kinetic constant for reaction 10 (gfp -->) was raised and lowered by an order of magnitude (from 3.21E-05 1/s). The base is shown for comparison:<br> | ||
| + | |||
| + | <center><gallery widths=300 heights=200> | ||
| + | Image:base.jpg|Figure 1: Base TTN Model | ||
| + | Image:gfp_u.jpg|Figure 2: Increased GFP Decomposition Rate (3.21E-04) | ||
| + | Image:gfp_d.jpg|Figure 3: Decreased GFP Decomposition Rate (3.21E-06) | ||
| + | </gallery></center><br> | ||
| + | |||
| + | As can be seen, there is a small difference when the GFP was increased, but no noticeable difference when GFP decomposition was decreased. The last model set GFP decomposition to zero in order to check whether a decrease would ever significantly affect the graph:<br> | ||
| + | |||
| + | <center><gallery widths=400 heights=300> | ||
| + | Image:gfp_d2.jpg|Figure 4: GFP Decomposition Set to Zero | ||
| + | </gallery></center><br> | ||
| + | |||
| + | As can be seen the elimination of GFP production doesn't have an effect. This makes sense since source literature says GFP decomposition is minor when compared to the dilution as a result of cell division. | ||
Latest revision as of 19:10, 30 July 2009
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Patrick
Today I received and analyzed the three models created yesterday, smad_ttn_gfp_u, smad_ttn_gfp_d, smad_ttn_gfp_d2. Below are the first two models, where the kinetic constant for reaction 10 (gfp -->) was raised and lowered by an order of magnitude (from 3.21E-05 1/s). The base is shown for comparison:
 Figure 1: Base TTN Model |
 Figure 2: Increased GFP Decomposition Rate (3.21E-04) |
 Figure 3: Decreased GFP Decomposition Rate (3.21E-06) |
As can be seen, there is a small difference when the GFP was increased, but no noticeable difference when GFP decomposition was decreased. The last model set GFP decomposition to zero in order to check whether a decrease would ever significantly affect the graph:
 Figure 4: GFP Decomposition Set to Zero |
As can be seen the elimination of GFP production doesn't have an effect. This makes sense since source literature says GFP decomposition is minor when compared to the dilution as a result of cell division.
