Team:Paris/Protocols PCRqload

From 2009.igem.org

(Difference between revisions)
(PCR with Quick load Taq2x Master Mix)
(PCR with Quick load Taq2x Master Mix)
 
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==PCR with Quick load Taq2x Master Mix==
==PCR with Quick load Taq2x Master Mix==
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*Mix for 50µl:
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*Mix for 50µl final:
**14µL H2O
**14µL H2O
**25µL Taq Quick load Master Mix 2X
**25µL Taq Quick load Master Mix 2X

Latest revision as of 00:02, 31 July 2009

PCR with Quick load Taq2x Master Mix

  • Mix for 50µl final:
    • 14µL H2O
    • 25µL Taq Quick load Master Mix 2X
    • 5µL Oligo Fw (0,2µM)
    • 5µL Oligo Rw (0,2µM)
    • 1µL Matrice (DNA)