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Team:LCG-UNAM-Mexico
From 2009.igem.org
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Bacteriophage infection represents a major problem in the industry and | Bacteriophage infection represents a major problem in the industry and | ||
research fields. The idea of being able to contend at a population | research fields. The idea of being able to contend at a population | ||
| - | level with such infections is | + | level with such infections is the main motivation for the development of our |
| - | + | project. We plan to modify an Escherichia coli phage in order to deliver | |
| - | project | + | genetic |
| - | + | ||
material codifying a construction in defense against other phages | material codifying a construction in defense against other phages | ||
taking advantage of some of the properties they can present. | taking advantage of some of the properties they can present. | ||
The purpose of this construction is that a bacterial population can | The purpose of this construction is that a bacterial population can | ||
manage to fight back the spreading of some phage by triggering on | manage to fight back the spreading of some phage by triggering on | ||
| - | + | a cellular death response when a cell encounters an | |
specific component of the phage. Such response will be faster than the | specific component of the phage. Such response will be faster than the | ||
| - | formation process of viral particles | + | formation process of viral particles preventing the death of the bacterial |
| - | the death of the bacterial population. This population resistance can | + | population. This population resistance can |
be seen as a sort of "vaccine" that will hold back the process of | be seen as a sort of "vaccine" that will hold back the process of | ||
infection. | infection. | ||
| Line 55: | Line 54: | ||
its auxiliary genes present in the P2 helper phage. In the case of the | its auxiliary genes present in the P2 helper phage. In the case of the | ||
"vaccine" construct, the cellular death response will be induced by | "vaccine" construct, the cellular death response will be induced by | ||
| - | the presence of T3 or T7 RNA polymerases which will also | + | the presence of T3 or T7 RNA polymerases which will also turn on the |
| - | + | transcription of toxines used for the | |
degradation of DNA and RNA to stop the phage´s genetic material from | degradation of DNA and RNA to stop the phage´s genetic material from | ||
assembling and scattering in the environment. Furthermore, we will | assembling and scattering in the environment. Furthermore, we will | ||
Revision as of 06:16, 2 August 2009
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Our ProjectBacteriophage infection represents a major problem in the industry and research fields. The idea of being able to contend at a population level with such infections is the main motivation for the development of our project. We plan to modify an Escherichia coli phage in order to deliver genetic material codifying a construction in defense against other phages taking advantage of some of the properties they can present. The purpose of this construction is that a bacterial population can manage to fight back the spreading of some phage by triggering on a cellular death response when a cell encounters an specific component of the phage. Such response will be faster than the formation process of viral particles preventing the death of the bacterial population. This population resistance can be seen as a sort of "vaccine" that will hold back the process of infection. The design of the delivery system includes the use the P4 phage and its auxiliary genes present in the P2 helper phage. In the case of the "vaccine" construct, the cellular death response will be induced by the presence of T3 or T7 RNA polymerases which will also turn on the transcription of toxines used for the degradation of DNA and RNA to stop the phage´s genetic material from assembling and scattering in the environment. Furthermore, we will implement a stochastic population model based on the basic properties of the bacterial cells and the phages such as movement, reproduction, etc., that will allow us to simulate the infection processes and quantify the efficiency of our system. A possible extensión of the system includes the expansion of an AHL signal through the quorum sensing system of Vibrio fischeri in which the population will be "warned" to prepare against the viral infection in the presence of T3 or T7. |
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