Team:Groningen/Alignment

From 2009.igem.org

(Difference between revisions)

Latest revision as of 13:06, 3 August 2009

The theoretical modeling data is used from papers is based on plasmids R46 and R773. To see the different between the chromosomal and the plasmids genes, they are aligned with [http://www.ebi.ac.uk/Tools/clustalw2/index.html clustalW]. The functional binding site from arsenic is on residues Cys32, Cys34, and the nonessential Cys37 (Rensing2005). Plasmids R46, R773, strain K12 and DH10B are aligned, and all have the same functional binding site on the same position. The distance between the genes is not significant. The assumption is that there is no difference in binding of arsenic and function between chromosomal and plasmids genes.

Alignment with CLUSTALW

Phylogram with CLUSTALW

Revision as of 12:34, 3 August 2009 (view source) Annelies (Talk \| contribs) ← Older edit		Latest revision as of 13:06, 3 August 2009 (view source) Jaspervdg (Talk \| contribs) m
(3 intermediate revisions not shown)
Line 1:		Line 1:
-	The theoretical modeling data is used from papers is based on plasmids R46 and R773. To see the different between the chromosomal and the plasmids genes, they are ~~alignment~~ with clustalW. The functional binding site from arsenic is on residues Cys32, Cys34, and the nonessential Cys37. Plasmids R46, R773, strain K12 and DH10B are ~~alignment~~, and all have the same functional binding site on the same position. The distance between the genes is not significant. We assumption that there no difference in binding in arsenic and function between chromosomal and plasmids genes.	+	The theoretical modeling data is used from papers is based on plasmids R46 and R773. To see the different between the chromosomal and the plasmids genes, they are aligned with [http://www.ebi.ac.uk/Tools/clustalw2/index.html clustalW]. The functional binding site from arsenic is on residues Cys32, Cys34, and the nonessential Cys37 ([[Team:Groningen/Literature#Rensing2005\|Rensing2005]]). Plasmids R46, R773, strain K12 and DH10B are aligned, and all have the same functional binding site on the same position. The distance between the genes is not significant. The assumption is that there is no difference in binding of arsenic and function between chromosomal and plasmids genes.