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Ann's Notebook
From 2009.igem.org
(Difference between revisions)
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[[media:7_27_09_Gel_of_RBS_and_Bacteriophage_lysis_cassette_digest_Plates_for_Pu_promoter_colony_PCR_Streaking_out_Pu_luciferase_part_from_the_registry.pdf| 7/27/09]] | [[media:7_27_09_Gel_of_RBS_and_Bacteriophage_lysis_cassette_digest_Plates_for_Pu_promoter_colony_PCR_Streaking_out_Pu_luciferase_part_from_the_registry.pdf| 7/27/09]] | ||
| + | *Plated overnight cultures for Pu colony PCR | ||
| + | *Protocol for running a gel | ||
| + | *Ran gel of the RBS for Pu promoter and Bacteriophage lysis cassette | ||
| + | *Plated Pu-luciferase part sent from the registry (Thanks!) | ||
Revision as of 23:25, 3 August 2009
- Media preparation for transformation
- Comp. cell protocol
- Protocol for getting parts from the biobricks
- Transformation protocol
- Transformation of RBS for Pu promoter, Bacteriophage lysis cassette, lambda lysis device, T4 lysis device
- Protocol for miniprep
- Miniprep of RBS for Pu promoter, Bacteriophage lysis cassette, lambda lysis device, T4 lysis device
- Protocol for using the nanodrop
- Protocol for a digestion
- Nanodrop concentrations of the RBS for Pu promoter, Bacteriophage lysis cassette, lambda lysis device, T4 lysis device
- Digestion of the RBS for Pu promoter and the Bacteriophage lysis cassette
- Plated overnight cultures for Pu colony PCR
- Protocol for running a gel
- Ran gel of the RBS for Pu promoter and Bacteriophage lysis cassette
- Plated Pu-luciferase part sent from the registry (Thanks!)
