"
Ann's Notebook
From 2009.igem.org
(Difference between revisions)
| Line 23: | Line 23: | ||
[[media:7_28_09_Frozen_stock_Pu_luciferase_and_digest_of_lambda_T4_killer_genes.pdf| 7/28/09]] | [[media:7_28_09_Frozen_stock_Pu_luciferase_and_digest_of_lambda_T4_killer_genes.pdf| 7/28/09]] | ||
| - | * | + | *Overnight culture for frozen stock of Pu-luciferase biobrick |
| - | *Digest of lambda and T4 killer genes to check part length | + | *Digest of lambda and T4 killer genes to check part length with EcoRI, PstI, BglII and EcoRI/BglII |
[[media:7_29_09_Frozen_stock_Pu_luciferase_gel_of_lambda_and_T4_digest_of_lambda_and_T4.pdf| 7/29/09]] | [[media:7_29_09_Frozen_stock_Pu_luciferase_gel_of_lambda_and_T4_digest_of_lambda_and_T4.pdf| 7/29/09]] | ||
| - | * | + | *Frozen stock of Pu-luciferase biobrick |
| + | *Gel of lambda and T4 killer genes digested with EcoRI, PstI, BglII and EcoRI/BglII | ||
Revision as of 23:46, 3 August 2009
- Media preparation for transformation
- Comp. cell protocol
- Protocol for getting parts from the biobricks
- Transformation protocol
- Transformation of RBS for Pu promoter, Bacteriophage lysis cassette, lambda lysis device, T4 lysis device
- Protocol for miniprep
- Miniprep of RBS for Pu promoter, Bacteriophage lysis cassette, lambda lysis device, T4 lysis device
- Protocol for using the nanodrop
- Protocol for a digestion
- Nanodrop concentrations of the RBS for Pu promoter, Bacteriophage lysis cassette, lambda lysis device, T4 lysis device
- Digestion of the RBS for Pu promoter and the Bacteriophage lysis cassette
- Plated overnight cultures for Pu colony PCR
- Protocol for running a gel
- Ran gel of the RBS for Pu promoter and Bacteriophage lysis cassette
- Plated Pu-luciferase part sent from the registry
- Overnight culture for frozen stock of Pu-luciferase biobrick
- Digest of lambda and T4 killer genes to check part length with EcoRI, PstI, BglII and EcoRI/BglII
- Frozen stock of Pu-luciferase biobrick
- Gel of lambda and T4 killer genes digested with EcoRI, PstI, BglII and EcoRI/BglII
