"
LOVTAP
From 2009.igem.org
(Difference between revisions)
| Line 3: | Line 3: | ||
==To do== | ==To do== | ||
| - | Write an e-mail to Strickland et al to ask: | + | ;Find the exact genetic circuit for Trp repressor |
| + | |||
| + | ;Write an e-mail to Strickland et al to ask: | ||
<br> - Sequence | <br> - Sequence | ||
<br> - Purification protocol | <br> - Purification protocol | ||
<br> - Digest assay protocol | <br> - Digest assay protocol | ||
| + | |||
| + | ;Biobricks: | ||
| + | <br> - Look for a (or many) paper(s) that characterizes E.Coli Trp repressor, and find the Trp operon sequence | ||
| + | <br> - Create a biobrick that coexpresses LOVTAP and mCherry (after Trp operon) when LOVTAP bind the Trp operon. | ||
Revision as of 17:46, 26 May 2009
To find
- Sequence of the 12 mutant fusion proteins => PUBMED (http://www.ncbi.nlm.nih.gov/)
To do
- Find the exact genetic circuit for Trp repressor
- Write an e-mail to Strickland et al to ask
- Sequence
- Purification protocol
- Digest assay protocol
- Biobricks
- Look for a (or many) paper(s) that characterizes E.Coli Trp repressor, and find the Trp operon sequence
- Create a biobrick that coexpresses LOVTAP and mCherry (after Trp operon) when LOVTAP bind the Trp operon.
| Home | The Team | The Project | Parts Submitted to the Registry | Modeling | Notebook | Lectures | Team Management |
|---|
