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LOVTAP
From 2009.igem.org
(Difference between revisions)
| Line 6: | Line 6: | ||
;Write an e-mail to Strickland et al to ask: | ;Write an e-mail to Strickland et al to ask: | ||
| - | + | -Sequence | |
<br> - Purification protocol | <br> - Purification protocol | ||
<br> - Digest assay protocol | <br> - Digest assay protocol | ||
;Biobricks: | ;Biobricks: | ||
| - | + | - Look for a (or many) paper(s) that characterizes E.Coli Trp repressor, and find the Trp operon sequence | |
<br> - Create a biobrick that coexpresses LOVTAP and mCherry (after Trp operon) when LOVTAP bind the Trp operon. | <br> - Create a biobrick that coexpresses LOVTAP and mCherry (after Trp operon) when LOVTAP bind the Trp operon. | ||
Revision as of 17:47, 26 May 2009
To find
- Sequence of the 12 mutant fusion proteins => PUBMED (http://www.ncbi.nlm.nih.gov/)
To do
- Find the exact genetic circuit for Trp repressor
- Write an e-mail to Strickland et al to ask
-Sequence
- Purification protocol
- Digest assay protocol
- Biobricks
- Look for a (or many) paper(s) that characterizes E.Coli Trp repressor, and find the Trp operon sequence
- Create a biobrick that coexpresses LOVTAP and mCherry (after Trp operon) when LOVTAP bind the Trp operon.
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