"
LOVTAP
From 2009.igem.org
(Difference between revisions)
| Line 5: | Line 5: | ||
;Find the exact genetic circuit for Trp repressor | ;Find the exact genetic circuit for Trp repressor | ||
| - | ;Write an e-mail to Strickland et al to ask | + | ;Write an e-mail to Strickland et al to ask |
-Sequence | -Sequence | ||
<br> - Purification protocol | <br> - Purification protocol | ||
<br> - Digest assay protocol | <br> - Digest assay protocol | ||
| - | ;Biobricks | + | ;Biobricks |
- Look for a (or many) paper(s) that characterizes E.Coli Trp repressor, and find the Trp operon sequence | - Look for a (or many) paper(s) that characterizes E.Coli Trp repressor, and find the Trp operon sequence | ||
<br> - Create a biobrick that coexpresses LOVTAP and mCherry (after Trp operon) when LOVTAP bind the Trp operon. | <br> - Create a biobrick that coexpresses LOVTAP and mCherry (after Trp operon) when LOVTAP bind the Trp operon. | ||
Revision as of 17:47, 26 May 2009
To find
- Sequence of the 12 mutant fusion proteins => PUBMED (http://www.ncbi.nlm.nih.gov/)
To do
- Find the exact genetic circuit for Trp repressor
- Write an e-mail to Strickland et al to ask
-Sequence
- Purification protocol
- Digest assay protocol
- Biobricks
- Look for a (or many) paper(s) that characterizes E.Coli Trp repressor, and find the Trp operon sequence
- Create a biobrick that coexpresses LOVTAP and mCherry (after Trp operon) when LOVTAP bind the Trp operon.
| Home | The Team | The Project | Parts Submitted to the Registry | Modeling | Notebook | Lectures | Team Management |
|---|
