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LOVTAP
From 2009.igem.org
(Difference between revisions)
| Line 6: | Line 6: | ||
;Write an e-mail to Strickland et al to ask | ;Write an e-mail to Strickland et al to ask | ||
| - | + | Sequence | |
| - | <br> | + | <br> Purification protocol |
| - | <br> | + | <br> Digest assay protocol |
;Biobricks | ;Biobricks | ||
| - | + | Look for a (or many) paper(s) that characterizes E.Coli Trp repressor, and find the Trp operon sequence | |
| - | <br> | + | <br> Create a biobrick that coexpresses LOVTAP and mCherry (after Trp operon) when LOVTAP bind the Trp operon. |
Revision as of 17:48, 26 May 2009
To find
- Sequence of the 12 mutant fusion proteins => PUBMED (http://www.ncbi.nlm.nih.gov/)
To do
- Find the exact genetic circuit for Trp repressor
- Write an e-mail to Strickland et al to ask
Sequence
Purification protocol
Digest assay protocol
- Biobricks
Look for a (or many) paper(s) that characterizes E.Coli Trp repressor, and find the Trp operon sequence
Create a biobrick that coexpresses LOVTAP and mCherry (after Trp operon) when LOVTAP bind the Trp operon.
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