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LOVTAP
From 2009.igem.org
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==To do== | ==To do== | ||
| - | ;Find the exact genetic circuit for Trp repressor | + | ;- Find the exact genetic circuit for Trp repressor |
| - | ;Write an e-mail to Strickland et al to ask | + | ;- Write an e-mail to Strickland et al to ask |
Sequence | Sequence | ||
<br> Purification protocol | <br> Purification protocol | ||
<br> Digest assay protocol | <br> Digest assay protocol | ||
| - | ;Biobricks | + | ;- Biobricks |
Look for a (or many) paper(s) that characterizes E.Coli Trp repressor, and find the Trp operon sequence | Look for a (or many) paper(s) that characterizes E.Coli Trp repressor, and find the Trp operon sequence | ||
<br> Create a biobrick that coexpresses LOVTAP and mCherry (after Trp operon) when LOVTAP bind the Trp operon. | <br> Create a biobrick that coexpresses LOVTAP and mCherry (after Trp operon) when LOVTAP bind the Trp operon. | ||
Revision as of 17:51, 26 May 2009
To find
- Sequence of the 12 mutant fusion proteins => PUBMED (http://www.ncbi.nlm.nih.gov/)
To do
- - Find the exact genetic circuit for Trp repressor
- - Write an e-mail to Strickland et al to ask
Sequence
Purification protocol
Digest assay protocol
- - Biobricks
Look for a (or many) paper(s) that characterizes E.Coli Trp repressor, and find the Trp operon sequence
Create a biobrick that coexpresses LOVTAP and mCherry (after Trp operon) when LOVTAP bind the Trp operon.
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