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Virginia Commonwealth/4 August 2009
From 2009.igem.org
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====Wetlab==== | ====Wetlab==== | ||
* New BioBrick Assembly Kit came in and the following were aliquoted into their respective amounts | * New BioBrick Assembly Kit came in and the following were aliquoted into their respective amounts | ||
| - | + | ** XbaI (10 µL) | |
| + | ** SpeI (5 µL) | ||
| + | ** PstI (10 µL) | ||
| + | ** EcoRI-HF (10 µL) | ||
| + | ** BSA (5 µL) | ||
| + | ** T4 Ligase Buffer (10 µL) | ||
| + | ** T4 Ligase (5 µL) | ||
''Maria and Afton'' | ''Maria and Afton'' | ||
* | * | ||
Revision as of 22:19, 4 August 2009
Contents |
Tuesday 4 August 2009
Results
Maria and Afton
- Overnight culture of I1352 plated on AMP:
- Sample taken from plate after PSB4C5 ligation attempt successfully grew expressing RFP. The color of the sample was pink
- Sample taken from plate after ligation with itself did not grow at all, and expressed no RFP
Trentay 22:11, 4 August 2009 (UTC)
Tasks
Maria and Afton
- Run a Gel Electrophoresis
- Transform and grow up pSB1C3 with the death gene P1010
Trentay 22:11, 4 August 2009 (UTC)
Wetlab
- New BioBrick Assembly Kit came in and the following were aliquoted into their respective amounts
- XbaI (10 µL)
- SpeI (5 µL)
- PstI (10 µL)
- EcoRI-HF (10 µL)
- BSA (5 µL)
- T4 Ligase Buffer (10 µL)
- T4 Ligase (5 µL)
Maria and Afton
