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Team:Paris/Freezer PCR products
From 2009.igem.org
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|width=10%| 274bp | |width=10%| 274bp | ||
|width=25%| Amplification of N-term domain of M13's g3p | |width=25%| Amplification of N-term domain of M13's g3p | ||
| - | |width=5%| | + | |width=5%| [https://2009.igem.org/Team:Paris/Fridge F4] |
|width=5%| ? ng/µL | |width=5%| ? ng/µL | ||
| - | |width=5%| | + | |width=5%| [https://2009.igem.org/Team:Paris/5_August_2009 08/05] |
|- style="background: #ccccff; text-align: center;" | |- style="background: #ccccff; text-align: center;" | ||
|width=5%| A2 | |width=5%| A2 | ||
| Line 68: | Line 68: | ||
|width=10%| [O16] TE3_Rw | |width=10%| [O16] TE3_Rw | ||
|width=5%| ? | |width=5%| ? | ||
| - | |width=10%| | + | |width=10%| [https://2009.igem.org/Team:Paris/Fridge F4] |
|width=10%| 1036bp | |width=10%| 1036bp | ||
|width=25%| Amplification of N-term domain of colicin E3 | |width=25%| Amplification of N-term domain of colicin E3 | ||
|width=5%| ? | |width=5%| ? | ||
|width=5%| ? ng/µL | |width=5%| ? ng/µL | ||
| - | |width=5%| | + | |width=5%| [https://2009.igem.org/Team:Paris/5_August_2009 08/05] |
|- style="background: #ccccff; text-align: center;" | |- style="background: #ccccff; text-align: center;" | ||
|width=5%| A3 | |width=5%| A3 | ||
| Line 80: | Line 80: | ||
|width=10%| [O10] clyA_fusion_polyG_Rw | |width=10%| [O10] clyA_fusion_polyG_Rw | ||
|width=5%| ? | |width=5%| ? | ||
| - | |width=10%| | + | |width=10%| [https://2009.igem.org/Team:Paris/Fridge F4] |
|width=10%| 987bp | |width=10%| 987bp | ||
|width=25%| Amplification of clyA with RBS in the primer | |width=25%| Amplification of clyA with RBS in the primer | ||
|width=5%| ? | |width=5%| ? | ||
|width=5%| ? ng/µL | |width=5%| ? ng/µL | ||
| - | |width=5%| | + | |width=5%| [https://2009.igem.org/Team:Paris/5_August_2009 08/05] |
|- style="background: #ccccff; text-align: center;" | |- style="background: #ccccff; text-align: center;" | ||
|width=5%| A4 | |width=5%| A4 | ||
| Line 92: | Line 92: | ||
|width=10%| [O12] ompA_signal_fusion_Rw | |width=10%| [O12] ompA_signal_fusion_Rw | ||
|width=5%| ? | |width=5%| ? | ||
| - | |width=10%| | + | |width=10%| [https://2009.igem.org/Team:Paris/Fridge F4] |
|width=10%| 135bp | |width=10%| 135bp | ||
|width=25%| Amplification of OmpA signal for export to periplasm | |width=25%| Amplification of OmpA signal for export to periplasm | ||
|width=5%| ? | |width=5%| ? | ||
|width=5%| ? ng/µL | |width=5%| ? ng/µL | ||
| - | |width=5%| | + | |width=5%| [https://2009.igem.org/Team:Paris/5_August_2009 08/05] |
|- style="background: #ccccff; text-align: center;" | |- style="background: #ccccff; text-align: center;" | ||
|width=5%| A5 | |width=5%| A5 | ||
| Line 104: | Line 104: | ||
|width=10%| [O13] tolR_II_Fusion_Fw | |width=10%| [O13] tolR_II_Fusion_Fw | ||
|width=5%| ? | |width=5%| ? | ||
| - | |width=10%| | + | |width=10%| [https://2009.igem.org/Team:Paris/Fridge F4] |
|width=10%| 279bp | |width=10%| 279bp | ||
|width=25%| Amplification of tolR | |width=25%| Amplification of tolR | ||
|width=5%| ? | |width=5%| ? | ||
|width=5%| ? ng/µL | |width=5%| ? ng/µL | ||
| - | |width=5%| | + | |width=5%| [https://2009.igem.org/Team:Paris/5_August_2009 08/05] |
|- style="background: #ccccff; text-align: center;" | |- style="background: #ccccff; text-align: center;" | ||
|width=5%| A6 | |width=5%| A6 | ||
| Line 116: | Line 116: | ||
|width=10%| [O5] Rev P5 | |width=10%| [O5] Rev P5 | ||
|width=5%| ? | |width=5%| ? | ||
| - | |width=10%| | + | |width=10%| [https://2009.igem.org/Team:Paris/Fridge F4] |
|width=10%| 1634bp | |width=10%| 1634bp | ||
|width=25%| Amplification of tatA with RBS | |width=25%| Amplification of tatA with RBS | ||
|width=5%| ? | |width=5%| ? | ||
|width=5%| ? ng/µL | |width=5%| ? ng/µL | ||
| - | |width=5%| | + | |width=5%| [https://2009.igem.org/Team:Paris/5_August_2009 08/05] |
|- style="background: #ccccff; text-align: center;" | |- style="background: #ccccff; text-align: center;" | ||
|width=5%| A? | |width=5%| A? | ||
Revision as of 19:30, 5 August 2009
Freezer
Primers - PCR products - Plasmids - Digestion Products - Unexpected digestion results - Strains (Glycerol stock)
| Color code for sticker | |||||||
|---|---|---|---|---|---|---|---|
| Vector | Insert | PCR product | Plasmid | Strains | Kanamycin | Ampicilin | Chloranphenicol |
PCR products
| PCR products | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Number | Name | Oligo F | Oligo R | Tm | Matrix | Length | Comments | Quantity | []ng/µL | Date |
| A1 | ? | [O18] Tg3p_fusion_Fw | [O17] Tg3p_Rw | ? | ? | 274bp | Amplification of N-term domain of M13's g3p | F4 | ? ng/µL | 08/05 |
| A2 | ? | [O15] TE3_fusion_Fw | [O16] TE3_Rw | ? | F4 | 1036bp | Amplification of N-term domain of colicin E3 | ? | ? ng/µL | 08/05 |
| A3 | ? | [O31] clyA_RBS_Fw | [O10] clyA_fusion_polyG_Rw | ? | F4 | 987bp | Amplification of clyA with RBS in the primer | ? | ? ng/µL | 08/05 |
| A4 | ? | [O11] ompA_signal_Fw | [O12] ompA_signal_fusion_Rw | ? | F4 | 135bp | Amplification of OmpA signal for export to periplasm | ? | ? ng/µL | 08/05 |
| A5 | ? | [O1] Rev P1 | [O13] tolR_II_Fusion_Fw | ? | F4 | 279bp | Amplification of tolR | ? | ? ng/µL | 08/05 |
| A6 | ? | [O14] tatABC_RBS_Fw | [O5] Rev P5 | ? | F4 | 1634bp | Amplification of tatA with RBS | ? | ? ng/µL | 08/05 |
| A? | g3p_std | [O19] g3p_F | [O20] g3p_R | 78°C | P12 | 1287bp | For standardisation of g3p and adding SacI site | ? | ? ng/µL | ? |
| A? | OmpA_L_std | [O21] Ompa_linker_F | [O22] Ompa_linker_R | 80°C | P6 | 525bp | For standardisation of OmpA linker (BBaK103006) | ? | ? ng/µL | ? |
http://parts.mit.edu/r/parts/images/partbypart/icon_terminator.png
http://parts.mit.edu/images/partbypart/icon_regulatory.png
http://parts.mit.edu/r/parts/images/partbypart/icon_rbs.png
http://parts.mit.edu/r/parts/images/partbypart/icon_reporter.png
http://parts.mit.edu/registry/images/7/7b/Part_icon_cds.png
http://parts.mit.edu/registry/images/9/9f/Part_icon_dna.png
http://parts.mit.edu/registry/images/2/2e/Part_icon_generator.png
