Ann's Notebook

From 2009.igem.org

(Difference between revisions)
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*Nanodrop of Pu promoter
*Nanodrop of Pu promoter
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[[media:8_5_09_Pu_promoter_colony_PCR_optimization,_Pu_promoter_purification_and_nanodrop.pdf| 8/5/09]]
+
[[media:8_6_09_Pu_Promoter_DNA_concentration_and_nanodrop.pdf| 8/6/09]]
 +
*Protocol for DNA precipitation
 +
*DNA concentration of Pu promoter
 +
*Nanodrop results for concentrated Pu promoter

Revision as of 01:59, 7 August 2009

6/19/09 and 6/25/09

  • M9 salts
  • Liquid LB
  • Antibiotic stock solutions
  • LB plates

7/22/09 and 7/23/09

  • Media preparation for transformation
  • Comp. cell protocol
  • Protocol for getting parts from the biobricks
  • Transformation protocol
  • Transformation of RBS for Pu promoter, Bacteriophage lysis cassette, lambda lysis device, T4 lysis device

7/24/09

  • Protocol for miniprep
  • Miniprep of RBS for Pu promoter, Bacteriophage lysis cassette, lambda lysis device, T4 lysis device

7/25/09

  • Protocol for using the nanodrop
  • Protocol for a digestion
  • Nanodrop concentrations of the RBS for Pu promoter, Bacteriophage lysis cassette, lambda lysis device, T4 lysis device
  • Digestion of the RBS for Pu promoter and the Bacteriophage lysis cassette

7/27/09

  • Plated overnight cultures for Pu colony PCR
  • Protocol for running a gel
  • Ran gel of the RBS for Pu promoter and Bacteriophage lysis cassette
  • Plated Pu-luciferase part sent from the registry

7/28/09

  • Overnight culture for frozen stock of Pu-luciferase biobrick
  • Digest of lambda and T4 killer genes to check part length with EcoRI, PstI, BglII and EcoRI/BglII

7/29/09

  • Frozen stock of Pu-luciferase biobrick
  • Gel of lambda and T4 killer genes digested with EcoRI, PstI, BglII and EcoRI/BglII
  • Digest of lambda and T4 killer genes with EcoRI/PstI, EcoRI/XhoI, XhoI

7/30/09

  • Gel of lambda and T4 killer genes digested with EcoRI/PstI, EcoRI/XhoI, XhoI
  • Gel of Pu promoter colony PCR

8/3/09

  • Analysis of Pu promoter colony PCR
    • Primer sequence
    • PCR conditions

8/4/09

  • Protocol for colony PCR
  • Colony PCR for Pu Promoter
  • Gel of Colony PCR for Pu promoter with samples at different annealing temperatures
  • Meeting with Pablo about HPLC

8/5/09

  • Protocol for DNA purification
  • DNA purification of Pu promoter colony PCR
  • Optimization of Pu promoter colony PCR
  • Nanodrop of Pu promoter

8/6/09

  • Protocol for DNA precipitation
  • DNA concentration of Pu promoter
  • Nanodrop results for concentrated Pu promoter
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