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LOVTAP
From 2009.igem.org
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Look for a (or many) paper(s) that characterizes E.Coli Trp repressor, and find the Trp operon sequence <font size=3><span style="color:orange"> Mél </span></font size> | Look for a (or many) paper(s) that characterizes E.Coli Trp repressor, and find the Trp operon sequence <font size=3><span style="color:orange"> Mél </span></font size> | ||
<br> Summary of what characterizes E.Coli Trp repressor : [[Media:The_tryptophan_biosynthetic_pathway.pdf]] | <br> Summary of what characterizes E.Coli Trp repressor : [[Media:The_tryptophan_biosynthetic_pathway.pdf]] | ||
| - | <br> The initial article : [[ | + | <br> The initial article : [[RNA-based_regulation_of_genes_of_tryptophan_synthesis_an_degradation.pdf]] |
<br> Sequence from NCBI : [[Media:Sequence_du_Trp_repressor.txt]] | <br> Sequence from NCBI : [[Media:Sequence_du_Trp_repressor.txt]] | ||
<br> Design a biobrick that coexpresses LOVTAP and mCherry (after Trp operon) when LOVTAP bind the Trp operon. Design a switch on/off read out. | <br> Design a biobrick that coexpresses LOVTAP and mCherry (after Trp operon) when LOVTAP bind the Trp operon. Design a switch on/off read out. | ||
Revision as of 19:17, 30 May 2009
To find
- Sequence of the 12 mutant fusion proteins or LOV domain AND TrpR (separately) => PUBMED (http://www.ncbi.nlm.nih.gov/)
To do
- - Find the exact genetic circuit for Trp repressor Nath
- - Write an e-mail to Strickland et al to ask Basile first shot; I think we could then look at it all together
Sequence (of all 12 fusion proteins)
Purification protocol
Digest assay protocol
- - Biobricks
Look for a (or many) paper(s) that characterizes E.Coli Trp repressor, and find the Trp operon sequence Mél
Summary of what characterizes E.Coli Trp repressor : Media:The_tryptophan_biosynthetic_pathway.pdf
The initial article : RNA-based_regulation_of_genes_of_tryptophan_synthesis_an_degradation.pdf
Sequence from NCBI : Media:Sequence_du_Trp_repressor.txt
Design a biobrick that coexpresses LOVTAP and mCherry (after Trp operon) when LOVTAP bind the Trp operon. Design a switch on/off read out.
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