Team:Paris/Freezer PCR products

From 2009.igem.org

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|width=5%| ?
|width=5%| ?
|width=10%| [https://2009.igem.org/Team:Paris/Fridge F4]
|width=10%| [https://2009.igem.org/Team:Paris/Fridge F4]
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|width=10%| 135bp
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|width=10%| 135
|width=25%| Amplification of OmpA signal for export to periplasm
|width=25%| Amplification of OmpA signal for export to periplasm
|width=5%| ?
|width=5%| ?
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|width=5%| ? ng/µL
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|width=5%| ?
|width=5%| [https://2009.igem.org/Team:Paris/5_August_2009#PCR 08/05]
|width=5%| [https://2009.igem.org/Team:Paris/5_August_2009#PCR 08/05]
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|width=5%| ?
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|width=10%| [https://2009.igem.org/Team:Paris/Fridge F4]
|width=10%| [https://2009.igem.org/Team:Paris/Fridge F4]
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|width=10%| 279bp
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|width=10%| 279
|width=25%| Amplification of tolR
|width=25%| Amplification of tolR
|width=5%| ?
|width=5%| ?
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|width=5%| ? ng/µL
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|width=5%| ?
|width=5%| [https://2009.igem.org/Team:Paris/5_August_2009#PCR 08/05]
|width=5%| [https://2009.igem.org/Team:Paris/5_August_2009#PCR 08/05]
|- style="background: #ccccff; text-align: center;"
|- style="background: #ccccff; text-align: center;"
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|width=5%| ?
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|width=10%| [https://2009.igem.org/Team:Paris/Fridge F4]
|width=10%| [https://2009.igem.org/Team:Paris/Fridge F4]
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|width=10%| 1634bp
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|width=10%| 1634
|width=25%| Amplification of tatA with RBS
|width=25%| Amplification of tatA with RBS
|width=5%| ?
|width=5%| ?
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|width=5%| ? ng/µL
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|width=5%| ?
|width=5%| [https://2009.igem.org/Team:Paris/5_August_2009#PCR 08/05]
|width=5%| [https://2009.igem.org/Team:Paris/5_August_2009#PCR 08/05]
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|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O19 O19 ]   
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O19 O19 ]   
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O20 O20 ]
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O20 O20 ]
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|width=5%| 78°C
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|width=5%| 78
|width=10%| P12
|width=10%| P12
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|width=10%| 1287bp
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|width=10%| 1287
|width=25%| For standardisation of g3p and adding SacI site
|width=25%| For standardisation of g3p and adding SacI site
|width=5%| ?
|width=5%| ?
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|width=5%| ? ng/µL
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|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O21 O21 ]   
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O21 O21 ]   
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O22 O22 ]
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O22 O22 ]
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|width=5%| 80°C
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|width=5%| 80
|width=10%| P6
|width=10%| P6
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|width=10%| 525bp
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|width=10%| 525
|width=25%| For standardisation of OmpA linker (BBaK103006)  
|width=25%| For standardisation of OmpA linker (BBaK103006)  
|width=5%| ?
|width=5%| ?
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|width=5%| ? ng/µL
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Revision as of 12:54, 9 August 2009

Freezer

Primers - PCR products - Plasmids - Digestion Products - Unexpected digestion results - Strains (Glycerol stock)


Color code for sticker
Vector Insert PCR product Plasmid Strains Kanamycin Ampicilin Chloranphenicol

PCR products

PCR products
Ref Name Oligo Fw Oligo Rev Tm used(Tm cal.) (°C) Matrix Length (bp) Comments Quantity [ ] (ng/µL) Date
A1 Tg3p_fusion N term O18 O17  ? (63.5) F4 274 Amplification of N-term domain of M13's g3p  ?  ? 08/05
A2 TE3_fusion N term O15 O16  ? F4 1036 Amplification of N-term domain of colicin E3  ?  ? 08/05
A3 clyA_fusion C term O31 O10  ?(55.5) F4 987 Amplification of clyA with RBS in the primer  ?  ? 08/05
A4 OmpAsignal_fusion C term O11 O12  ? F4 135 Amplification of OmpA signal for export to periplasm  ?  ? 08/05
A5 tolRII_fusion N term O13 O1  ? F4 279 Amplification of tolR  ?  ? 08/05
A6 tatABC_RBS O14 O15  ? F4 1634 Amplification of tatA with RBS  ?  ? 08/05
A? g3p_std O19 O20 78 P12 1287 For standardisation of g3p and adding SacI site  ?  ?  ?
A? OmpA_L_std O21 O22 80 P6 525 For standardisation of OmpA linker (BBaK103006)  ?  ?  ?



http://parts.mit.edu/r/parts/images/partbypart/icon_terminator.png http://parts.mit.edu/images/partbypart/icon_regulatory.png http://parts.mit.edu/r/parts/images/partbypart/icon_rbs.png http://parts.mit.edu/r/parts/images/partbypart/icon_reporter.png http://parts.mit.edu/registry/images/7/7b/Part_icon_cds.png http://parts.mit.edu/registry/images/9/9f/Part_icon_dna.png http://parts.mit.edu/registry/images/2/2e/Part_icon_generator.png http://parts.mit.edu/registry/images/a/ab/Part_icon_plasmid.png

Retrieved from "http://2009.igem.org/Team:Paris/Freezer_PCR_products"