Team:Paris/Fridge

From 2009.igem.org

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! colspan="10" style="background: #ccccff;"|Fridge tube
! colspan="10" style="background: #ccccff;"|Fridge tube
|- style="background: #ccccff; text-align: center;"
|- style="background: #ccccff; text-align: center;"
-
|width=5%| Number
+
|width=5%| Ref
-
|width=10%| Name
+
|width=40%| Name
-
|width=25%| Comments
+
|width=40%| Comments
 +
|width=5%| Quantity
 +
|width=5%| Storage
|width=5%| Date
|width=5%| Date
 +
 +
|- style="background: #ccccff; text-align: left;"
 +
|F1
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|[[https://2009.igem.org/Team:Paris/Freezer_Strains_%28Glycerol_stock%29#Freezer#Strain_1 S1]] DH5alpha
 +
|Overnight culture for Glycerol Stock
 +
|Ø
 +
|
 +
|[[Team:Paris/21_July_2009 | 07/21]]
 +
 +
|- style="background: #ccccff; text-align: left;"
 +
|F2
 +
|[[https://2009.igem.org/Team:Paris/Strains#Strain_2 S2]] K12 MG 1655 delta recA::Kan
 +
|Overnight culture for Glycerol Stock
 +
|Ø
 +
|
 +
|[[Team:Paris/22_July_2009 | 07/21]]
 +
 +
|- style="background: #ccccff; text-align: left;"
 +
|F3
 +
|[[https://2009.igem.org/Team:Paris/Strains#Strain_1 S1]] DH5alpha
 +
|Overnight culture for competent bacteria
 +
|Ø
 +
|
 +
|[[Team:Paris/22_July_2009 | 07/22]]
 +
 +
|- style="background: #ccccff; text-align: left;"
 +
|F4
 +
|Genomic DNA of [[https://2009.igem.org/wiki/Team:Paris/Strains#Strain_2 S2]] K12 MG 1655 delta recA::Kan
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|Genomic DNA Purification
 +
|
 +
|4°C
 +
|[[Team:Paris/22_July_2009 | 07/22]]
 +
 +
|- style="background: #ccccff; text-align: left;"
 +
|F5
 +
|Stock CaCl<sub>2</sub> 0,1M
 +
|Filtred the 07/23
 +
|400ml
 +
|4°C
 +
|[[Team:Paris/23_July_2009 | 07/23]]
 +
 +
|- style="background: #ccccff; text-align: left;"
 +
|F5.1
 +
|CaCl<sub>2</sub> 0,1M
 +
|Filtred the 07/23
 +
|25ml
 +
|4°C
 +
|[[Team:Paris/23_July_2009 | 07/23]]
 +
 +
|- style="background: #ccccff; text-align: left;"
 +
|F5.2
 +
|CaCl<sub>2</sub> 0,1M
 +
|Filtred the 07/23
 +
|25ml
 +
|4°C
 +
|[[Team:Paris/23_July_2009 | 07/23]]
 +
 +
|- style="background: #ccccff; text-align: left;"
 +
|F5.3
 +
|CaCl<sub>2</sub> 0,1M
 +
|Filtred the 07/23
 +
|25ml
 +
|4°C
 +
|[[Team:Paris/23_July_2009 | 07/23]]
 +
 +
|- style="background: #ccccff; text-align: left;"
 +
|F5.4
 +
|CaCl<sub>2</sub> 0,1M
 +
|Filtred the 07/23
 +
|5ml
 +
|4°C
 +
|[[Team:Paris/23_July_2009 | 07/23]]
 +
 +
|- style="background: #ccccff; text-align: left;"
 +
|F6
 +
|[[https://2009.igem.org/Team:Paris/Strains#Strain_1 S1]] DH5alpha
 +
|Overnight culture for competent bacteria
 +
|Ø
 +
|
 +
|[[Team:Paris/23_July_2009 | 07/23]]
 +
 +
|- style="background: #ccccff; text-align: left;"
 +
|F7
 +
|CaCl<sub>2</sub> 50mM
 +
|Filtred the 07/23
 +
|18ml
 +
|4°C
 +
|[[Team:Paris/23_July_2009 | 07/23]]
 +
 +
|- style="background: #ccccff; text-align: left;"
 +
|F8
 +
|DH5 alpha competent
 +
|by CaCl<sub>2</sub>
 +
|2ml
 +
|4°C
 +
|[[Team:Paris/23_July_2009 | 07/23]]
 +
 +
|- style="background: #ccccff; text-align: left;"
 +
|F9
 +
|Buffer I
 +
|For SuperCompetent Bacteria Protocol
 +
|250ml
 +
|4°C
 +
|[[Team:Paris/27_July_2009 | 07/27]]
 +
 +
|- style="background: #ccccff; text-align: left;"
 +
|F10
 +
|Buffer II
 +
|For SuperCompetent Bacteria Protocol
 +
|125ml
 +
|4°C
 +
|[[Team:Paris/27_July_2009 | 07/27]]
 +
 +
|- style="background: #ccccff; text-align: left;"
 +
|Fx
 +
|x
 +
|x
 +
|
 +
|4°C
 +
|[[Team:Paris/xx_July_2009 | 07/xx]]
 +
|}
|}

Latest revision as of 13:13, 9 August 2009


Fridge tube

Fridge tube
Ref Name Comments Quantity Storage Date
F1 [S1] DH5alpha Overnight culture for Glycerol Stock Ø 07/21
F2 [S2] K12 MG 1655 delta recA::Kan Overnight culture for Glycerol Stock Ø 07/21
F3 [S1] DH5alpha Overnight culture for competent bacteria Ø 07/22
F4 Genomic DNA of [S2] K12 MG 1655 delta recA::Kan Genomic DNA Purification 4°C 07/22
F5 Stock CaCl2 0,1M Filtred the 07/23 400ml 4°C 07/23
F5.1 CaCl2 0,1M Filtred the 07/23 25ml 4°C 07/23
F5.2 CaCl2 0,1M Filtred the 07/23 25ml 4°C 07/23
F5.3 CaCl2 0,1M Filtred the 07/23 25ml 4°C 07/23
F5.4 CaCl2 0,1M Filtred the 07/23 5ml 4°C 07/23
F6 [S1] DH5alpha Overnight culture for competent bacteria Ø 07/23
F7 CaCl2 50mM Filtred the 07/23 18ml 4°C 07/23
F8 DH5 alpha competent by CaCl2 2ml 4°C 07/23
F9 Buffer I For SuperCompetent Bacteria Protocol 250ml 4°C 07/27
F10 Buffer II For SuperCompetent Bacteria Protocol 125ml 4°C 07/27
Fx x x 4°C 07/xx

Retrieved from "http://2009.igem.org/Team:Paris/Fridge"