Team:Paris/Freezer PCR products

From 2009.igem.org

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|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O16 O16 ]
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O16 O16 ]
|width=5%| ?
|width=5%| ?
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|width=10%| [https://2009.igem.org/Team:Paris/Fridge F4]
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|width=10%| ???
|width=10%| 1036
|width=10%| 1036
|width=25%| Amplification of N-term domain of colicin E3
|width=25%| Amplification of N-term domain of colicin E3
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|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O11 O11 ]   
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O11 O11 ]   
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O12 O12 ]
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O12 O12 ]
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|width=5%| ?
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|width=5%| ?(70.4)
|width=10%| [https://2009.igem.org/Team:Paris/Fridge F4]
|width=10%| [https://2009.igem.org/Team:Paris/Fridge F4]
|width=10%| 135
|width=10%| 135
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|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O13 O13 ]   
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O13 O13 ]   
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O1 O1 ]
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O1 O1 ]
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|width=5%| ?
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|width=5%| (73.5)
|width=10%| [https://2009.igem.org/Team:Paris/Fridge F4]
|width=10%| [https://2009.igem.org/Team:Paris/Fridge F4]
|width=10%| 279
|width=10%| 279
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|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O14 O14 ]   
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O14 O14 ]   
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O15 O15 ]
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O15 O15 ]
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|width=5%| ?
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|width=5%| (63.5)
|width=10%| [https://2009.igem.org/Team:Paris/Fridge F4]
|width=10%| [https://2009.igem.org/Team:Paris/Fridge F4]
|width=10%| 1634
|width=10%| 1634
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|width=5%| [https://2009.igem.org/Team:Paris/5_August_2009#PCR 08/05]
|width=5%| [https://2009.igem.org/Team:Paris/5_August_2009#PCR 08/05]
|- style="background: #ccccff; text-align: center;"
|- style="background: #ccccff; text-align: center;"
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|width=5%| A?
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|width=5%| A7 <span/ id="A7">
|width=10%| g3p_std
|width=10%| g3p_std
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O19 O19 ]   
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O19 O19 ]   
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O20 O20 ]
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O20 O20 ]
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|width=5%| 78
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|width=5%| (52)
|width=10%| P12
|width=10%| P12
|width=10%| 1287
|width=10%| 1287
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|width=5%| ?
|width=5%| ?
|width=5%| ?
|width=5%| ?
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|width=5%| ?
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|width=5%| [https://2009.igem.org/Team:Paris/8_August_2009#PCR 08/08]
|- style="background: #ccccff; text-align: center;"
|- style="background: #ccccff; text-align: center;"
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|width=5%| A?
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|width=5%| A8 <span/ id="A8">
|width=10%| OmpA_L_std
|width=10%| OmpA_L_std
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O21 O21 ]   
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O21 O21 ]   
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O22 O22 ]
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O22 O22 ]
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|width=5%| 80
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|width=5%| (59.5)
|width=10%| P6
|width=10%| P6
|width=10%| 525
|width=10%| 525
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|width=5%| ?
|width=5%| ?
|width=5%| ?
|width=5%| ?
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|- style="background: #ccccff; text-align: center;"
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|width=5%| A9 <span/ id="A9">
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|width=10%| Tg3p_fusion N term
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|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O18 O18 ] 
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|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O17 O17 ]
 +
|width=5%| (63.5)
 +
|width=10%| [https://2009.igem.org/Team:Paris/Fridge F4]
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|width=10%| 274
 +
|width=25%| Amplification of N-term domain of M13's g3p
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|width=5%| ?
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|width=5%| ?
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|width=5%| [https://2009.igem.org/Team:Paris/7_August_2009#PCR 08/07]
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Revision as of 14:37, 9 August 2009

Freezer

Primers - PCR products - Plasmids - Digestion Products - Unexpected digestion results - Strains (Glycerol stock)


Color code for sticker
Vector Insert PCR product Plasmid Strains Kanamycin Ampicilin Chloranphenicol

PCR products

PCR products
Ref Name Oligo Fw Oligo Rev Tm used(Tm cal.) (°C) Matrix Length (bp) Comments Quantity [ ] (ng/µL) Date
A1 Tg3p_fusion N term O18 O17  ? (63.5) F4 274 Amplification of N-term domain of M13's g3p  ?  ? 08/05
A2 TE3_fusion N term O15 O16  ?  ??? 1036 Amplification of N-term domain of colicin E3  ?  ? 08/05
A3 clyA_fusion C term O31 O10  ?(55.5) F4 987 Amplification of clyA with RBS in the primer  ?  ? 08/05
A4 OmpAsignal_fusion C term O11 O12  ?(70.4) F4 135 Amplification of OmpA signal for export to periplasm  ?  ? 08/05
A5 tolRII_fusion N term O13 O1 (73.5) F4 279 Amplification of tolR  ?  ? 08/05
A6 tatABC_RBS O14 O15 (63.5) F4 1634 Amplification of tatA with RBS  ?  ? 08/05
A7 g3p_std O19 O20 (52) P12 1287 For standardisation of g3p and adding SacI site  ?  ? 08/08
A8 OmpA_L_std O21 O22 (59.5) P6 525 For standardisation of OmpA linker (BBaK103006)  ?  ?  ?
A9 Tg3p_fusion N term O18 O17 (63.5) F4 274 Amplification of N-term domain of M13's g3p  ?  ? 08/07



http://parts.mit.edu/r/parts/images/partbypart/icon_terminator.png http://parts.mit.edu/images/partbypart/icon_regulatory.png http://parts.mit.edu/r/parts/images/partbypart/icon_rbs.png http://parts.mit.edu/r/parts/images/partbypart/icon_reporter.png http://parts.mit.edu/registry/images/7/7b/Part_icon_cds.png http://parts.mit.edu/registry/images/9/9f/Part_icon_dna.png http://parts.mit.edu/registry/images/2/2e/Part_icon_generator.png http://parts.mit.edu/registry/images/a/ab/Part_icon_plasmid.png

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