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Virginia Commonwealth/29 June 2009
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''Maria and Afton'' | ''Maria and Afton'' | ||
* There were no results from last night, since the cells were grown up in the wrong E. Coli. | * There were no results from last night, since the cells were grown up in the wrong E. Coli. | ||
| + | [[User:MandM|MandM]] 19:41, 29 June 2009 (UTC) | ||
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''Clay and Craig'' | ''Clay and Craig'' | ||
* There was no growth on the plate from last night's transformations. The wrong strain of E.coli was used. It did not have the death gene resistance. | * There was no growth on the plate from last night's transformations. The wrong strain of E.coli was used. It did not have the death gene resistance. | ||
| + | ---- | ||
===Tasks=== | ===Tasks=== | ||
''Maria and Afton'' | ''Maria and Afton'' | ||
* Last night we had grown up the backbone PSB3K3 in E. Coli Strain NEB10β, however since it contained P1010 we should have used E. Coli strain DB3.1. | * Last night we had grown up the backbone PSB3K3 in E. Coli Strain NEB10β, however since it contained P1010 we should have used E. Coli strain DB3.1. | ||
| + | [[User:MandM|MandM]] 19:41, 29 June 2009 (UTC) | ||
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''Clay and Craig'' | ''Clay and Craig'' | ||
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** E. Coli Cells Zapped on LB (+) | ** E. Coli Cells Zapped on LB (+) | ||
[[User:MandM|MandM]] 19:41, 29 June 2009 (UTC) | [[User:MandM|MandM]] 19:41, 29 June 2009 (UTC) | ||
| + | |||
''Clay and Craig'' | ''Clay and Craig'' | ||
* Kevin made fresh LB+agar and Craig poured and labeled the plates. | * Kevin made fresh LB+agar and Craig poured and labeled the plates. | ||
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''Kevin and Adam'' | ''Kevin and Adam'' | ||
Latest revision as of 15:43, 10 August 2009
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Contents |
Monday 29 June 2009
Results
Maria and Afton
- There were no results from last night, since the cells were grown up in the wrong E. Coli.
MandM 19:41, 29 June 2009 (UTC)
Clay and Craig
- There was no growth on the plate from last night's transformations. The wrong strain of E.coli was used. It did not have the death gene resistance.
Tasks
Maria and Afton
- Last night we had grown up the backbone PSB3K3 in E. Coli Strain NEB10β, however since it contained P1010 we should have used E. Coli strain DB3.1.
MandM 19:41, 29 June 2009 (UTC)
Clay and Craig
- Redo last nights transformations.
- Make new LB plates.
Wetlab
Maria and Afton
- Today we replated cells containing PSB3K3 in E. Coli Strain DB3.1.
- The controls for the experiment are:
- E. Coli Cells on LB (+)
- E. Coli Cells on LB with KAN (-)
- E. Coli Cells Zapped on LB (+)
MandM 19:41, 29 June 2009 (UTC)
Clay and Craig
- Kevin made fresh LB+agar and Craig poured and labeled the plates.
Kevin and Adam
