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Team:Groningen/Parts
From 2009.igem.org
(Difference between revisions)
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|Ampicillin | |Ampicillin | ||
|plate 3, pos.29 | |plate 3, pos.29 | ||
| + | |Restriction(+) | ||
| + | |- | ||
| + | |CueO- + GVP in J61035 | ||
| + | |CueO promoter without RBS in front of the GVP cluster | ||
| + | |None | ||
| + | |6149 | ||
| + | |[http://partsregistry.org/wiki/index.php?title=Part:BBa_J61035 BBa_J61035] (without the part between EcoRI and XbaI) | ||
| + | |Ampicillin | ||
| + | |plate 3, pos.37 | ||
| + | |Restriction(+) | ||
| + | |- | ||
| + | |ZntR- + GVP in J61035 | ||
| + | |ZntR promoter without RBS in front of the GVP cluster | ||
| + | |None | ||
| + | |~6150 | ||
| + | |[http://partsregistry.org/wiki/index.php?title=Part:BBa_J61035 BBa_J61035] (without the part between EcoRI and XbaI) | ||
| + | |Ampicillin | ||
| + | |plate 3, pos.38 | ||
| + | |Restriction(+) | ||
| + | |- | ||
| + | |ArsR- + GVP in J61035 | ||
| + | |ArsR promoter without RBS in front of the GVP cluster | ||
| + | |None | ||
| + | |~6150 | ||
| + | |[http://partsregistry.org/wiki/index.php?title=Part:BBa_J61035 BBa_J61035] (without the part between EcoRI and XbaI) | ||
| + | |Ampicillin | ||
| + | |plate 3, pos.39 | ||
|Restriction(+) | |Restriction(+) | ||
|} | |} | ||
Revision as of 08:58, 11 August 2009
[http://2009.igem.org/Team:Groningen http://2009.igem.org/wiki/images/f/f1/Igemhomelogo.png]
| Biobricks
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Note
If you choose to include a Parts Submitted to the Registry page, please list your parts here. This is not necessary but it may be a nice list to keep track of.
- http://partsregistry.org/wiki/images/d/db/Buttonaddpart.gif
[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=Groningen Our sandbox]
Part List -80
| Component | Description | Part or Accession # | Base Pairs (bp) | Plasmid (backbone) | Resistance | Well | Quality control |
| GVP | Gas Vesicle Proteins | [http://partsregistry.org/Part:BBa_I750016 BBa_I750016] | 6064 | [http://partsregistry.org/Part:BBa_J61035 BBa_J61035] | Ampicillin/Gentamycin | plate 3, pos.1 | ? |
| GVP (2nd batch) | Gas Vesicle Proteins | [http://partsregistry.org/Part:BBa_I750016 BBa_I750016] | 6064 | [http://partsregistry.org/Part:BBa_J61035 BBa_J61035] | Ampicillin/Gentamycin | plate 3, pos.2 | ? |
| pHigh in low copy nr vector | High consitutive promoter | 35bp | Kanamycin | Plate 3, pos. 4 | PCR(?), restriction(+) | ||
| pMed in low copy nr vector | Medium consitutive promoter | 35bp | Kanamycin | Plate 3, pos. 5 | PCR(?), restriction(+) | ||
| pLow in low copy nr vector | Low consitutive promoter | 35bp | Kanamycin | Plate 3, pos. 6 | PCR(?), restriction(+) | ||
| pHigh in high copy nr vector | High consitutive promoter | 35bp | Ampicillin/Chloramphenicol | Plate 3, pos. 7 | PCR(?), restriction(+) | ||
| pMed in high copy nr vector | Medium consitutive promoter | 35bp | Ampicillin/Chloramphenicol | Plate 3, pos. 8 | PCR(?), restriction(+) | ||
| pLow in high copy nr vector | High consitutive promoter | 35bp | Ampicillin/Chloramphenicol | Plate 3, pos. 9 | PCR(?), restriction(+) | ||
| Promotor (low expression) | Constitutive promotor | [http://partsregistry.org/Part:BBa_J23109 BBa_J23109] | 35 | [http://partsregistry.org/Part:BBa_J61002 BBa_J61002] | Ampicillin | plate 3, pos.10 | No |
| Promotor (medium expression) | Constitutive promotor | [http://partsregistry.org/Part:BBa_J23106 BBa_J23106] | 35 | [http://partsregistry.org/Part:BBa_J61002 BBa_J61002] | Ampicillin | plate 3, pos.11 | No |
| Promotor (high expression) | Constitutive promotor | [http://partsregistry.org/Part:BBa_J23100 BBa_J23100] | 35 | [http://partsregistry.org/Part:BBa_J61002 BBa_J61002] | Ampicillin | plate 3, pos.12 | No |
| Inducible promoter | Lactose inducible promoter nr1 | 200bp | Ampicillin | Plate 3, pos. 14 | PCR(?), restriction(+) | ||
| Inducible promoter | Lactose inducible promoter nr2 | 200bp | Ampicillin | Plate 3, pos. 15 | PCR(?), restriction(+) | ||
| RBS | Ribosomal Binding Site | [http://partsregistry.org/Part:BBa_B0034 BBa_B0034] | 12 | [http://partsregistry.org/Part:pSB1A2 pSB1A2] | Ampicillin | plate 3, pos.19 | No |
| Terminator | Double terminator consisting of [http://partsregistry.org/Part:BBa_B0012 BBa_B0012] and [http://partsregistry.org/Part:BBa_B0011 BBa_B0011] | [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] | ~90 | [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] | Ampicillin/Kanamycin | plate 3, pos.20 | No |
| HmtA (1.1) | HmtA-His in pBAD(a) | None | ? | None | Ampicillin | plate 3, pos.28 | Restriction(+) |
| HmtA (2.1) | HmtA-His in pBAD(a) | None | ? | None | Ampicillin | plate 3, pos.29 | Restriction(+) |
| CueO- + GVP in J61035 | CueO promoter without RBS in front of the GVP cluster | None | 6149 | [http://partsregistry.org/wiki/index.php?title=Part:BBa_J61035 BBa_J61035] (without the part between EcoRI and XbaI) | Ampicillin | plate 3, pos.37 | Restriction(+) |
| ZntR- + GVP in J61035 | ZntR promoter without RBS in front of the GVP cluster | None | ~6150 | [http://partsregistry.org/wiki/index.php?title=Part:BBa_J61035 BBa_J61035] (without the part between EcoRI and XbaI) | Ampicillin | plate 3, pos.38 | Restriction(+) |
| ArsR- + GVP in J61035 | ArsR promoter without RBS in front of the GVP cluster | None | ~6150 | [http://partsregistry.org/wiki/index.php?title=Part:BBa_J61035 BBa_J61035] (without the part between EcoRI and XbaI) | Ampicillin | plate 3, pos.39 | Restriction(+) |
