Team:UNICAMP-Brazil/Notebooks/July 25

From 2009.igem.org

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These are the primers that we´re going to use in out project. How long will it take until they´re sent to us?? :)
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Col-R CTACTAGTACCAGAAATTCAGCCTCGGTA
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Anticol-F TTCTAGAGGTGGTAGGGAACGCTTTGAA
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Anticol-R CTACTAGTAACCGGCATTCATATTTCAGC
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HLY-F TTCTAGAGATAAAGATGGTCGAGA
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HLY-R CTACTAGTCTAACGTTCACGTAAACTT
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LYS-F TTCTAGAATGGTTGAAATCAATAATC
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LYS-R CTACTAGTTACATCAATCTCTCTGACC
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FinOF-XbaI TTCTAGAGATGACAGAGCAGAAACGACCGG
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FinOR-SpeI CTACTAGTATTATTTCTCATCAAGCACGGCC
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FinPF-XbaI TTCTAGAGTGACGAGCATGTTTTTGTTGAA
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FinPR-SpeI CTACTAGTAAAAATCGCCGATGCAGGGAGAC
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Ppy-F-1 TTCTAGAGACGAGAAGGCTATGTGTATCA
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Ppy-F-2 TTCTAGAGTTTCCGTCTATTTACCTTTTCTG
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Ppy-R CTACTAGTATAACACCTCCCGCTGTTTAT
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KlJEN1-F TTCTAGATCAACCATGAATAACAACAAT
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KlJEN1-R CTACTAGTATAATGCTATGGTGCGTGT
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Prom-KlJEN1-F TTCTAGATGATTGGCAAACCTGTTGT
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Prom-KlJEN1-R CTACTAGTGGTTGAAATCTGTTTTGGTTTT
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Prom-KlDLD-F TTCTAGAGGTCGCTCTTTCTCTCTCTCAT
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Prom-KlDLD-R CTACTAGTTGAGTTGCGTTTCTGGTCTTT
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GgLYSO_F TTCTAGAATGAGGTCTTTGCTAATCTTGG
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GgLYSO_R CTACTAGTTTTTAATGAGGGATGCGTTT
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Cre-F TCCAATTTACTGACCGTACACC
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FSTK_UP_VF2 CACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTTGCCACCTGACGTCTAAGAA
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FSTK_UP_VR TACAGGCTTTTCCTGCCCGATGCTAAAACGGACAACAGTACCATTACCGCCTTTGAGTGAGC
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FSTK_DOWN_VF2 TCGTATGCCGGATAAGGCGCGGTAGCGTCGCATCCGGCACTCTGCCACCTGACGTCTAAGAA
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FSTK_DOWN_VR AAATTACGTCACTCCCGATCGGGAGTTCCCTCTACTCTCTATATTACCGCCTTTGAGTGAGC
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Slippage_Cre-R TGGTGTACGGTCAGTAAATTGGAGACTGACTGACTGACTGACTGACTGACTGACTGACTGACTGACTCAT

Revision as of 15:50, 12 August 2009

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These are the primers that we´re going to use in out project. How long will it take until they´re sent to us?? :)


Col-R CTACTAGTACCAGAAATTCAGCCTCGGTA

Anticol-F TTCTAGAGGTGGTAGGGAACGCTTTGAA

Anticol-R CTACTAGTAACCGGCATTCATATTTCAGC

HLY-F TTCTAGAGATAAAGATGGTCGAGA

HLY-R CTACTAGTCTAACGTTCACGTAAACTT

LYS-F TTCTAGAATGGTTGAAATCAATAATC

LYS-R CTACTAGTTACATCAATCTCTCTGACC

FinOF-XbaI TTCTAGAGATGACAGAGCAGAAACGACCGG

FinOR-SpeI CTACTAGTATTATTTCTCATCAAGCACGGCC

FinPF-XbaI TTCTAGAGTGACGAGCATGTTTTTGTTGAA

FinPR-SpeI CTACTAGTAAAAATCGCCGATGCAGGGAGAC

Ppy-F-1 TTCTAGAGACGAGAAGGCTATGTGTATCA

Ppy-F-2 TTCTAGAGTTTCCGTCTATTTACCTTTTCTG

Ppy-R CTACTAGTATAACACCTCCCGCTGTTTAT

KlJEN1-F TTCTAGATCAACCATGAATAACAACAAT

KlJEN1-R CTACTAGTATAATGCTATGGTGCGTGT

Prom-KlJEN1-F TTCTAGATGATTGGCAAACCTGTTGT

Prom-KlJEN1-R CTACTAGTGGTTGAAATCTGTTTTGGTTTT

Prom-KlDLD-F TTCTAGAGGTCGCTCTTTCTCTCTCTCAT

Prom-KlDLD-R CTACTAGTTGAGTTGCGTTTCTGGTCTTT

GgLYSO_F TTCTAGAATGAGGTCTTTGCTAATCTTGG

GgLYSO_R CTACTAGTTTTTAATGAGGGATGCGTTT

Cre-F TCCAATTTACTGACCGTACACC

FSTK_UP_VF2 CACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTTGCCACCTGACGTCTAAGAA

FSTK_UP_VR TACAGGCTTTTCCTGCCCGATGCTAAAACGGACAACAGTACCATTACCGCCTTTGAGTGAGC

FSTK_DOWN_VF2 TCGTATGCCGGATAAGGCGCGGTAGCGTCGCATCCGGCACTCTGCCACCTGACGTCTAAGAA

FSTK_DOWN_VR AAATTACGTCACTCCCGATCGGGAGTTCCCTCTACTCTCTATATTACCGCCTTTGAGTGAGC

Slippage_Cre-R TGGTGTACGGTCAGTAAATTGGAGACTGACTGACTGACTGACTGACTGACTGACTGACTGACTGACTCAT