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Virginia Commonwealth/4 August 2009
From 2009.igem.org
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* Possible inefficiency of BioBrick enzymes may have come from repeated freezing and thawing | * Possible inefficiency of BioBrick enzymes may have come from repeated freezing and thawing | ||
** They will be aliquoted into smaller amounts | ** They will be aliquoted into smaller amounts | ||
| + | * Gel Electrophoresis Results | ||
| + | [[Image:2009-08-04 DigestI1352andpSB4C5.jpg|350px|thumb|none| From Left to Right: 100bp Ladder, Bioline Hyperladder, I1352(Digest), I1352(Miniprep), pSB4C5(Digest), pSB4C5(Miniprep)]] | ||
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[[User:Trentay|Trentay]] 22:25, 4 August 2009 (UTC) | [[User:Trentay|Trentay]] 22:25, 4 August 2009 (UTC) | ||
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===Tasks=== | ===Tasks=== | ||
''Maria and Afton'' | ''Maria and Afton'' | ||
Revision as of 18:27, 13 August 2009
Contents |
Tuesday 4 August 2009
Results
Maria and Afton
- Overnight culture of I1352 plated on AMP:
- Sample taken from plate after PSB4C5 ligation attempt successfully grew expressing RFP. The color of the sample was pink
- Sample taken from plate after ligation with itself did not grow at all, and expressed no RFP
- There is a possibility the pSB4C5 backbone may be a bad part
- previous years it has not functioned properly
- Gel electrophoresis indicates DNA Gel Purification may be necessary
- Possible inefficiency of BioBrick enzymes may have come from repeated freezing and thawing
- They will be aliquoted into smaller amounts
- Gel Electrophoresis Results
Trentay 22:25, 4 August 2009 (UTC)
Tasks
Maria and Afton
- Run a Gel Electrophoresis
- Transform and grow up pSB1C3 with the death gene P1010
Trentay 22:11, 4 August 2009 (UTC)
Wetlab
- New BioBrick Assembly Kit came in and the following were aliquoted into their respective amounts
| Name | Amount/Tube | # Tubes | Extra in Stock | |
|---|---|---|---|---|
| XbaI | 10 µL | 15 | No | |
| SpeI | 5 µL | 10 | No | |
| PstI | 10 µL | 25 | Yes | |
| EcoRI-HF | 10 µL | 25 | Yes | |
| BSA | 5 µL | 8 | Yes | |
| T4 Ligase Buffer | 10 µL | 25 | Yes | |
| T4 Ligase | 10 µL | 8 | No |
- To show how many times a tube has been thawed and refrozen, a hash mark will be placed on the side of the tube, each time it is thawed as a reference for others to see
Trentay 15:24, 5 August 2009 (UTC)
Maria and Afton
- Gel Electrophoresis was run
- pSB4C5 and I1352 digested and undigested (right after Miniprep) were run on a gel and results showed excessive bands in the undigested DNA. DNA Gel Purification will be done on future Miniprepped DNA
- pSB1C3 w/ P1010 was removed from Well 5E on Plate 1 of the Distribution Kit and resuspended in 10µL of TE Buffer.
- Transformation was done using DB 3.1
| Name | Plate | Growth Observation | |
|---|---|---|---|
| pSB1C3 | CM | n/a | |
| (+) Control | LB | n/a | |
| (+) Control zapped | LB | n/a | |
| (-) Control | CM | n/a |
Trentay 22:33, 4 August 2009 (UTC)
