August/12 August 2009

From 2009.igem.org

(Difference between revisions)

Revision as of 08:02, 14 August 2009

1.before Min prep
Checked the cell cultures transformed and plated out yesterday (8/10) for colony formation and made an approximate count of the number of colonies.

(plate number)-(location on plate) (no. of colonies)
             1-14F                      50<
             1-14H                      50<
             1-23J                       100~
             1-12C                       100~
             2-18F                       10

inoculate to iquid medium(5mL + Amp 5uL or Kan 25uL)

2.Transformation
to get new plasmid , and ligation sample

each 4uL(DNA) , 1-2M , 1-12C : 2uL
1-2M Amp
2-8O
1-21F
2-21F
1-12A
2-8I
1-12C
2-24G
1-16G

1-18L Kan
2-11N

'8/11sample  10uL
RBS+2-8M(LasR)
EpsE+Terminater
(after 65℃ , 10min)

3.LB plate

LB(500uL)+Amp(2.5mL)  *25
                  +Kan(0.5mL)  *25

4.Min prep
Result of Nanodrop concentration check

(sample No.) (concentration)
1-15N            19.0 ng/uL 
2-6P               27.0 
1-18C             21.5 
1-12H             48.0
1-23L             23.7
1-23L               19.4
1-6I                   9.3
 
1-20F                 71.3
2-18F                 19.2
1-14H                 49.9
1-23J                   21.3
1-12C                  10.7
1-14F                  32

@@ Line 15: / Line 15: @@
 .<B>Transformation</b><br>
-to get new plasmid
+to get new plasmid , and ligation sample
   each 4uL(DNA) , 1-2M , 1-12C : 2uL
 -2M Amp