From 2009.igem.org

(Difference between revisions)

Revision as of 10:06, 16 August 2009

parts planning

teble sample

Vector	Insert
name	name
link to BBa~	link to BBa~
total size (bp)	insert size (bp)
Plasmid

sensor

signal sensor

3O6HSL P(tetR)+luxI

1.RBS + luxI

Vector	Insert
RBS	luxI
[http://partsregistry.org/Part:BBa_B0034 BBa_B0034]	[http://partsregistry.org/Part:BBa_C0161 BBa_C0161]
2091bp	585bp
pSB1A2

2.RBS+ luxI + terminator

Vector	Insert
double terminator	RBS+luxI
[http://partsregistry.org/Part:BBa_B0015 BBa_B0015]
4554bp	579bp
pSB1AK3

3.P(tetR)+RBS+ luxI + terminator

Vector	Insert
BBa_R0040	RBS+luxI+terminator
[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]
2133bp	726bp
pSB1A2

C4HSL P(tetR)+rhlI

1.RBS + rhII

Vector	Insert
RBS	rhII
pSB1A2

2.RBS + rhII + terminator

Vector	Insert
double terminator	RBS + rhII
[http://partsregistry.org/Part:BBa_B0015 BBa_B0015]
pSB1AK3

3.P(tetR) + RBS + rhlI + terminator

Vector	Insert
p(tetR)	RBS + rhII +terminator
[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]
pSB1A2

AI-1 P(tetR)+lasI

1.RBS + LasI

Vector	Insert
RBS	LasI
pSB1A2

2.RBS + LasI +terminator

Vector	Insert
double terminator	RBS + LasI +terminator
[http://partsregistry.org/Part:BBa_B0015 BBa_B0015]
pSB1AK3

3.P(tetR) + RBS + LasI +terminator

Vector	Insert
p(tetR)	RBS + LasI +terminator
[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]
pSB1A2

3OH,C14:1-HSL P(tetR)+cinI

1. RBS + cinI

Vector	Insert
RBS	cinI
pSB1A2

2.RBS + cinI +terminator

Vector	Insert
double terminator	RBS + cinI +terminator
[http://partsregistry.org/Part:BBa_B0015 BBa_B0015]
pSB1AK3

3.P(tetR) + RBS + cinI +terminator

Vector	Insert
p(tetR)	RBS + cinI +terminator
[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]
pSB1A2

receiver

signal receiver

3O6HSL luxpL+luxR+luxpR

[http://partsregistry.org/Part:BBa_F2621 BBa_F2621](1158bp)
pSB1A2

C4HSL p(tetR) + rhlR + RhlR+C4

1.RhlR+p(Rhl)

Vector	Insert
RhlR	p(RhlR)
[http://partsregistry.org/Part:BBa_I1466 BBa_I1466]	[http://partsregistry.org/Part:BBa_I14017 BBa_I14017]
pSB2K3

2.p(tetR)+RhlR+p(Rhl)

Vector	Insert
p(tetR)	RhlR+p(Rhl)
[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]
pSB1A2

AI-1 p(tetR) + lasR + LasR+pAI

1. RBS + lasR

Vector	Insert
RBS	lasR
pSB1A2

2.RBS + lasR +terminator

Vector	Insert
double terminator	RBS + lasR +terminator
[http://partsregistry.org/Part:BBa_B0015 BBa_B0015]
pSB1AK3

3.P(tetR) + RBS + lasR +terminator

Vector	Insert
p(tetR)	RBS + lasR +terminator
[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]
pSB1A2

4.P(tetR) + RBS + lasR +terminator +lasR+pAI

Vector	Insert
lasR+pAI	P(tetR) + RBS + lasR +terminator
[http://partsregistry.org/Part:BBa_R0079 BBa_R0079]
pSB1A2

3OH,C14:1-HSL p(tetR) + cinR + cinR

1. RBS + cinR

Vector	Insert
RBS	cinR
pSB1A2

2.RBS + cinR +terminator

Vector	Insert
double terminator	RBS + cinR +terminator
[http://partsregistry.org/Part:BBa_B0015 BBa_B0015]
pSB1AK3

3.P(tetR) + RBS + cinR +terminator

Vector	Insert
p(tetR)	RBS + cinR +terminator
[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]
pSB1A2

4.P(tetR) + RBS + cinR +terminator +p(cinR)

Vector	Insert
p(cinR)	P(tetR) + RBS + cinR +terminator
[http://partsregistry.org/Part:BBa_R0078 BBa_R0078]
pSB1A2

@@ Line 22: / Line 22: @@
 <tr><td>RBS</td><td>luxI</td></tr>
 <tr><td>[http://partsregistry.org/Part:BBa_B0034 BBa_B0034]</td><td>[http://partsregistry.org/Part:BBa_C0161 BBa_C0161]</td><tr>
+<tr><td>2091bp</td><td>585bp</td></tr>
 <tr><td>pSB1A2</td><td> </td></tr>
 </table>
@@ Line 31: / Line 32: @@
 <tr><td>double terminator</td><td>RBS+luxI</td></tr>
 <tr><td>[http://partsregistry.org/Part:BBa_B0015 BBa_B0015]</td><td> </td></tr>
+<tr><td>4554bp</td><td>579bp</td></tr>
 <tr><td>pSB1AK3</td><td></td></tr>
 </table>
@@ Line 40: / Line 42: @@
 <tr><td>BBa_R0040</td><td>RBS+luxI+terminator</td></tr>
 <tr><td>[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]</td><td> </td></tr>
+<tr><td>2133bp</td><td>726bp</td></tr>
 <tr><td>pSB1A2</td><td> </td></tr>
 </table>
 =='''C4HSL'''   P(tetR)+rhlI<br>==

August/16 August 2009