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Team:UNC Chapel Hill/17 August 2009
From 2009.igem.org
(Difference between revisions)
(New page: 1. Digested A8 (destination plasmid) 2. Ran Ethanol Precipitation on A8 (it uses PstI) For tomorrow: Run Gels of A1, A3, A8, and ladder if you can find it. A1, A3, and A8 are at the end ...) |
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1. Digested A8 (destination plasmid) | 1. Digested A8 (destination plasmid) | ||
2. Ran Ethanol Precipitation on A8 (it uses PstI) | 2. Ran Ethanol Precipitation on A8 (it uses PstI) | ||
| + | 3. Ran Gel of A1 (new and old), A3 (new and old), A8 (new and undigested), and Ladder | ||
| + | |||
For tomorrow: | For tomorrow: | ||
| - | |||
| - | |||
Ligate A1, A3, A8. | Ligate A1, A3, A8. | ||
Transform the result. | Transform the result. | ||
Latest revision as of 22:15, 17 August 2009
1. Digested A8 (destination plasmid) 2. Ran Ethanol Precipitation on A8 (it uses PstI) 3. Ran Gel of A1 (new and old), A3 (new and old), A8 (new and undigested), and Ladder
For tomorrow:
Ligate A1, A3, A8. Transform the result.
