August/21 August 2009
From 2009.igem.org
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===restriction and digestion=== | ===restriction and digestion=== | ||
- | + | ⅰ パーツ⑨ | |
- | + | Insert | |
- | + | H2O 4 | |
- | + | No.2 buffer 2 | |
- | + | ①ー3 12 | |
- | + | EcoRI 1 | |
+ | SpeⅠ |
Revision as of 00:44, 22 August 2009
transformation
to get new plasmid
- ⑤(RBS+rhlR+terminator+promoter(reg))
- ⑦(RBS+cinR)
- ⑧(RBS+luxI)
check the result of transformation
2-8G 4 2-21F 0 2-6O 2 2-13N <15 ⑤ 0
restriction and digestion
ⅰ パーツ⑨ Insert H2O 4 No.2 buffer 2 ①ー3 12 EcoRI 1 SpeⅠ