Team:Virginia/3 June 2009

From 2009.igem.org

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==Today==
==Today==
*Mini-Prep of Factor 7.
*Mini-Prep of Factor 7.
-
Repeat of growth curve standards.
+
*Repeat of growth curve standards.
 +
**k12
 +
**DBH5-alpha
 +
*Glycerol stocks (15%) for -80 freezer of
 +
**JW3469-1 (arsB knockout)
 +
**JW3470-1 (arsC knockout)
 +
*Set up dilutions of DH5a E.coli to get OD v cell count calibration
 +
{| border="1" align="center"
 +
|+Cell Count of Serial 1:10 dilutions
 +
|Plate Num.
 +
|Optical Density
 +
|Original Concentration (X unknown)
 +
|1:10^1
 +
|1:10^2
 +
|1:10^3
 +
|1:10^4
 +
|1:10^5
 +
|1:10^6
 +
|1:10^7
 +
|1:10^8
 +
|----
 +
|1
 +
|0.57
 +
|X
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|----
 +
|2
 +
|0.41
 +
|.75X
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|----
 +
|3
 +
|0.27
 +
|.5X
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|----
 +
|4
 +
|0.1
 +
|.25X
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|----
 +
|5
 +
|0
 +
|.1X
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|
 +
|----
 +
|}
 +
 
 +
 
 +
*Another digest of A09-1 (X,P)

Latest revision as of 20:49, 3 June 2009

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Overnight Results

Results of plating

  • psB1T3 Nothing
  • psB1C3 Small colonies
  • psB4A5 lawn
  • psB1A3 lawn
  • psB1K3 Small colonies

Today

  • Mini-Prep of Factor 7.
  • Repeat of growth curve standards.
    • k12
    • DBH5-alpha
  • Glycerol stocks (15%) for -80 freezer of
    • JW3469-1 (arsB knockout)
    • JW3470-1 (arsC knockout)
  • Set up dilutions of DH5a E.coli to get OD v cell count calibration
Cell Count of Serial 1:10 dilutions
Plate Num. Optical Density Original Concentration (X unknown) 1:10^1 1:10^2 1:10^3 1:10^4 1:10^5 1:10^6 1:10^7 1:10^8
1 0.57 X
2 0.41 .75X
3 0.27 .5X
4 0.1 .25X
5 0 .1X


  • Another digest of A09-1 (X,P)
Retrieved from "http://2009.igem.org/Team:Virginia/3_June_2009"