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Template:Team:KULeuven/21 August 2009/VanillinProduction
From 2009.igem.org
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* Test 2: We loaded yesterday's restrictions on an agarose gel. It showed that the enzymes cut properly. | * Test 2: We loaded yesterday's restrictions on an agarose gel. It showed that the enzymes cut properly. | ||
| - | However, the EF results were | + | However, the EF results were unexpected. It seems that somehow the bacteria had taken in a self-closed Ech-plasmid. The bands do not correspond with an EF-ligation product. |
* The DNA was purified from the gel | * The DNA was purified from the gel | ||
Revision as of 07:13, 24 August 2009
- Test 2: We loaded yesterday's restrictions on an agarose gel. It showed that the enzymes cut properly.
However, the EF results were unexpected. It seems that somehow the bacteria had taken in a self-closed Ech-plasmid. The bands do not correspond with an EF-ligation product.
- The DNA was purified from the gel
Nanodrop results:
| Part | ng/µl | 260/280 | |
|---|---|---|---|
| Sam8 | 2,5 | 1,14 | |
| Sam5 | 7,9 | 1,60 | |
| Ech | 5,0 | 1,38 | |
| Fcs | 3,4 | 0,93 |
- Sam8 and Sam5; Fcs and Ech were ligated
Used volumes:
| Part | µl | |
|---|---|---|
| Sam8 | 34 | |
| Sam5 | 6,3 | |
| Ech | 10,0 | |
| Fcs | 35,8 |
