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Modelling
From 2009.igem.org
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| - | <span style="color:red">We will follow the following article protocol: | + | <span style="color:red">We will follow the following article protocol:</span> |
<br> Freddolino, P.L., Dittrich M., Schulten K., Dynamic Switching Mechanisms in LOV1 and LOV2 Domains of Plant Phototropins. Biophysical Journal, 91, 3630-3639, 2006 ([http://www.ncbi.nlm.nih.gov/sites/entrez?db=pmc&cmd=search&term=PMC1630464 Pubmed]) | <br> Freddolino, P.L., Dittrich M., Schulten K., Dynamic Switching Mechanisms in LOV1 and LOV2 Domains of Plant Phototropins. Biophysical Journal, 91, 3630-3639, 2006 ([http://www.ncbi.nlm.nih.gov/sites/entrez?db=pmc&cmd=search&term=PMC1630464 Pubmed]) | ||
Revision as of 21:11, 3 June 2009
To find
PDB files of LOV domain, TrpR repressor => [http://www.rcsb.org/pdb/home/home.do PDB database]
To do
OK - Set up day of starting modeling, do a planning for modeling => see with Matteo Dal Peraro ----> will be setted up as soon as the exams are over
Ask Matteo to send all the required starting papers, files, and links to tutorials BEFORE the end of the exams (around midjune)
- - Model allosteric interactions between LOVTAP & TrpR
- What will be done:
- -> Model of LOVTAP in dark phase
- -> Model of LOVTAP in light phase
- -> Characterize how the J-alpha helix changes
- -> Model sturctural changes that enhance the switch feature of LOVTAP e.g. in dark phase: really weak interaction between LOVTAP and the corresponding DNA sequence, in light phase: strong binding of LOVTAP on DNA.
We will follow the following article protocol:
Freddolino, P.L., Dittrich M., Schulten K., Dynamic Switching Mechanisms in LOV1 and LOV2 Domains of Plant Phototropins. Biophysical Journal, 91, 3630-3639, 2006 ([http://www.ncbi.nlm.nih.gov/sites/entrez?db=pmc&cmd=search&term=PMC1630464 Pubmed])
- - Model the fusion of LOV domain with other transcription factors
To envisage
- Molecular mutationnal assay
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