1. PCR product of 12-august of pairt
2. Miniprep of

Nanodrop results:

1. Restriction digest cut with EcoRI and XbaI
2. Electroporation of + and the ligation of ...
3. Gelelectrophoresis of
   • Only 1 band, so probably everything was cut
4. Gelextraction of
   • Nanodrop = 29,3 ng/μl
5. Ligation of vector and insert

1. Plated COMT (+RBS) from the registry
   • Growing overnight in 37°C
2. Plated ech
   • From newly made liquid culture
3. Miniprep of ech and sam8
   • Nanodrop results:

1. Restriction digest on ech and sam8
2. Gel electrophoresis on ech and sam8
3. New electrocompetent cells were used for the electroporation with the old ligations
   • Lig A -> sam5 + sam8
   • Lig C -> ech + fcs
   • Note: there was no spark
4. Plating of the electroporated cells
   • 200 μl per plate twice
   • 600 μl left of A and C

1. B and R miniprepped. Tomorrow proceed with mutagenesis of RpoA
2. G redone (only TOPO was visible, 200bp) PCR from 2,3 and 1'
3. W, X, Y, Z PCR purification to create a TOPO vector tomorrow

Nanodrop: