"
Uppsala-Sweden/26 August 2009
From 2009.igem.org
(Difference between revisions)
m (New page: {{Uppsala-Sweden Template}} ==Evaluating Competence== The results from yesterdays evaluation was disappointing. Only Hsin Ho's cells had any transformants at all. This seems a bit implaus...) |
|||
| (One intermediate revision not shown) | |||
| Line 2: | Line 2: | ||
==Evaluating Competence== | ==Evaluating Competence== | ||
| - | The results from yesterdays evaluation was disappointing. Only Hsin Ho's cells had any transformants at all. This seems a bit implausible so we performed.... | + | The results from yesterdays evaluation was disappointing. Only Hsin Ho's cells had any transformants at all. This seems a bit implausible so we performed a new evaluation, this time employing only our recently made Top10 cells. We inoculated using 1 and 5 µl of the pUC19 evaluation plasmid. |
| + | |||
| + | --[[User:Anders|Anders]] 20:33, 26 August 2009 (UTC) | ||
| + | |||
| + | ==Ethanol and Butanol Evolution== | ||
| + | |||
| + | I transferred the Ethanol culture from #3 ( 2min UV exposure ) to 5% ethanol in 15ml BG11. A control with 0% was setup as well. | ||
| + | |||
| + | Furthermore I inocultated recovery culutures from 0,25%, 0,5% and 1 % in 1,5% Butanol BG11 medium(15ml total volume). | ||
| + | A control with 0% Butanol was done as well | ||
| + | |||
| + | --[[User:Karl.brune|Karl.brune]] 15:07, 26 August 2009 (UTC) | ||
Latest revision as of 20:33, 26 August 2009
Evaluating Competence
The results from yesterdays evaluation was disappointing. Only Hsin Ho's cells had any transformants at all. This seems a bit implausible so we performed a new evaluation, this time employing only our recently made Top10 cells. We inoculated using 1 and 5 µl of the pUC19 evaluation plasmid.
--Anders 20:33, 26 August 2009 (UTC)
Ethanol and Butanol Evolution
I transferred the Ethanol culture from #3 ( 2min UV exposure ) to 5% ethanol in 15ml BG11. A control with 0% was setup as well.
Furthermore I inocultated recovery culutures from 0,25%, 0,5% and 1 % in 1,5% Butanol BG11 medium(15ml total volume). A control with 0% Butanol was done as well
--Karl.brune 15:07, 26 August 2009 (UTC)
