Ann's Notebook

From 2009.igem.org

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Revision as of 03:27, 27 August 2009


Ann's Notebook


6/19/09 and 6/25/09

  • M9 salts
  • Liquid LB
  • Antibiotic stock solutions
  • LB plates

7/22/09 and 7/23/09

  • Media preparation for transformation
  • Comp. cell protocol
  • Protocol for getting parts from the biobricks
  • Transformation protocol
  • Transformation of RBS for Pu promoter, Bacteriophage lysis cassette, lambda lysis device, T4 lysis device

7/24/09

  • Protocol for miniprep
  • Miniprep of RBS for Pu promoter, Bacteriophage lysis cassette, lambda lysis device, T4 lysis device

7/25/09

  • Protocol for using the nanodrop
  • Protocol for a digestion
  • Nanodrop concentrations of the RBS for Pu promoter, Bacteriophage lysis cassette, lambda lysis device, T4 lysis device
  • Digestion of the RBS for Pu promoter and the Bacteriophage lysis cassette

7/27/09

  • Plated overnight cultures for Pu colony PCR
  • Protocol for running a gel
  • Ran gel of the RBS for Pu promoter and Bacteriophage lysis cassette
  • Plated Pu-luciferase part sent from the registry

7/28/09

  • Overnight culture for frozen stock of Pu-luciferase biobrick
  • Digest of lambda and T4 killer genes to check part length with EcoRI, PstI, BglII and EcoRI/BglII

7/29/09

  • Frozen stock of Pu-luciferase biobrick
  • Gel of lambda and T4 killer genes digested with EcoRI, PstI, BglII and EcoRI/BglII
  • Digest of lambda and T4 killer genes with EcoRI/PstI, EcoRI/XhoI, XhoI

7/30/09

  • Gel of lambda and T4 killer genes digested with EcoRI/PstI, EcoRI/XhoI, XhoI
  • Gel of Pu promoter colony PCR

8/3/09

  • Analysis of Pu promoter colony PCR
    • Primer sequence
    • PCR conditions

8/4/09

  • Protocol for colony PCR
  • Colony PCR for Pu Promoter
  • Gel of Colony PCR for Pu promoter with samples at different annealing temperatures
  • Meeting with Pablo about HPLC

8/5/09

  • Protocol for DNA purification
  • DNA purification of Pu promoter colony PCR
  • Optimization of Pu promoter colony PCR
  • Nanodrop of Pu promoter

8/6/09

  • Protocol for DNA precipitation
  • DNA concentration of Pu promoter
  • Nanodrop results for concentrated Pu promoter

8/8/09

  • Comp. cell preparation and transformation of a GFP generator

8/9/09

  • Pu promoter colony PCR round 2
  • Gel of Pu promoter colony PCR round 2
  • DNA purification of colony PCR round 2
  • Nanodrop of colony PCR round 2 and leu landing pad
  • Miniprep of GFP generator
  • Nanodrop of GFP generator
  • Digest for Pu-RBS-GFP

8/10/09

  • Gel of digest for Pu-RBS-GFP

8/11/09

  • Digest of GFP generator
  • Gel of GFP generator
  • Pu promoter colony PCR round 3
  • Gel of Pu promoter colony PCR round 3
  • DNA purification of Pu promoter colony PCR round 3
  • Nanodrop of Pu promoter colony PCR round 3

8/12/09

  • Pu promoter round 3 digestion
  • DNA purification of digest for Pu-RBS-GFP
  • Nanodrop of digest for Pu-RBS-GFP

8/12/09

  • Gel of purified DNA for digest round 1
  • Digest of DNA for Pu-RBS-GFP ligation round 2
  • Trouble shooting DNA purification process
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