Team:KULeuven/Modelling/Blue Light Receptor

From 2009.igem.org

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=Blue light Sensor=
=Blue light Sensor=
==Overview==
==Overview==
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The purpose of the sensor is to increase the production of the RIBOKEY under the control of the intensity of the incident blue light.
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The purpose of the sensor is to enter the wanted vanillin concentration. Under the influence of the intensity of a blue light, the RIBOKEY is transcribed. The light used to perform the test has a wavelength of 470nm.
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Input: Blue light (<math>\lambda = 470</math>) <br>
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The biology works as follows:  
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Output: mRNA_RIBOKEY
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[[Image:black_box_light_sensor.JPG|center|thumb|300px|Sensor interface]]
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YcgF protein is believed to dimerize upon photo-excitation. The YcgE protein functions as a repressor for the transcription of the mRNA_RIBOKEY. However, the dimer form of YcgF is capable of interacting with the operator-bound YcgE, thereby releasing it from the DNA and starting transcription.
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[[Image:Biologie_blue_light.png|center]]
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The biology works as follows, YcgF protein is believed to dimerize upon photo-excitation.
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The YcgE protein is a repressor for the transcription of the mRNA_RIBOKEY, only the dimer form of YcgF is capable of  
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interacting with the operator-bound YcgE and to release it from the DNA.
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==Models==
The entire network of interactions is visualized in the diagram below
The entire network of interactions is visualized in the diagram below
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[[Image:Bls.JPG|center|thumb|600px|Regulation of the production of mRNA key by incident blue light]]
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[[Image:blue_light_sensor.jpg|center|thumb|630px|Regulation of the production of mRNA key by incident blue light]]
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==Reactions==
==Reactions==
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YcgF(Blrp), a Blue-Light Sensing Protein That Uses FAD (BLUF)"
YcgF(Blrp), a Blue-Light Sensing Protein That Uses FAD (BLUF)"
[http://pubs.acs.org/doi/abs/10.1021/bi051820x]
[http://pubs.acs.org/doi/abs/10.1021/bi051820x]
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"Influence of a Joining Helix on the BLUF Domain of the
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YcgF Photoreceptor from Escherichia coli"
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[http://www3.interscience.wiley.com/cgi-bin/fulltext/121407023/PDFSTART]
===YcgF - YcgE interaction===
===YcgF - YcgE interaction===

Latest revision as of 14:01, 28 August 2009

Contents

Blue light Sensor

Overview

The purpose of the sensor is to enter the wanted vanillin concentration. Under the influence of the intensity of a blue light, the RIBOKEY is transcribed. The light used to perform the test has a wavelength of 470nm.

The biology works as follows:

YcgF protein is believed to dimerize upon photo-excitation. The YcgE protein functions as a repressor for the transcription of the mRNA_RIBOKEY. However, the dimer form of YcgF is capable of interacting with the operator-bound YcgE, thereby releasing it from the DNA and starting transcription.

Biologie blue light.png

Models

The entire network of interactions is visualized in the diagram below

Regulation of the production of mRNA key by incident blue light

Reactions

Dimerization of YcgF

"Transient Dimerization and Conformational Change of a BLUF Protein: YcgF", [http://pubs.acs.org/doi/full/10.1021/ja065682q]

Sensor interface

"Light Induced Structural Changes of a Full-length Protein and Its BLUF Domain in YcgF(Blrp), a Blue-Light Sensing Protein That Uses FAD (BLUF)" [http://pubs.acs.org/doi/abs/10.1021/bi051820x]

"Influence of a Joining Helix on the BLUF Domain of the YcgF Photoreceptor from Escherichia coli" [http://www3.interscience.wiley.com/cgi-bin/fulltext/121407023/PDFSTART]

YcgF - YcgE interaction

"The BLUF-EAL protein YcgF acts as a direct anti-repressor in a blue-light response of Escherichia coli" [http://genesdev.cshlp.org/content/23/4/522.full.pdf+html]

Transcription of the RIBOKEY

https://2008.igem.org/Team:KULeuven/Model/Filter

Degradation of the RIBOKEY

https://2008.igem.org/Team:KULeuven/Model/Filter

References

[1] Natalia Tschowri, Susan Busse and Regine Hengge, "The BLUF-EAL protein YcgF acts as a direct anti-repressor in a blue-light response of Escherichia coli"

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