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Team:UNIPV-Pavia/Notebook/Week4Aug
From 2009.igem.org
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**B7(E-P) | **B7(E-P) | ||
**B8(E-P) | **B8(E-P) | ||
| - | **A14(E-P) | + | **A14-3(E-P) |
| - | **A16(E-P) | + | **A16-4(E-P) |
**I714891(E-P) | **I714891(E-P) | ||
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| + | *Gel results: | ||
| + | **B7 - both screened colonies were negative (~3200bp of vector and ~3200bp of insert). | ||
| + | **B8 - only B8-5 shows the expected length for ligated plasmid (~3200bp of vector and ~4200bp of insert), but it also shows a single unexpected band...All the other 4 samples were negative (~3200bp of vector and ~3200bp of insert). | ||
| + | **A14-3 - showed the expected length for ligated plasmid (2079bp of vector and ~2200bp of insert). | ||
| + | **A16-4 - showed the expected length for ligated plasmid (2079bp of vector and ~1800bp of insert). | ||
| + | **I714891 - showed the expected length for ligated plasmid (~2700bp of vector and ~700bp of insert). | ||
Revision as of 15:29, 29 August 2009
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Week from August 24th, to August 30th, 2009
Previous Week
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Next Week
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August, 24th
- Miniprep for B7 (2 samples), B8 (5 samples), A14-3, A16-4 and I714891. Bacterial pellets were stored at -20°C.
- Screening digestion for all the miniprepped samples:
- B7(E-P)
- B8(E-P)
- A14-3(E-P)
- A16-4(E-P)
- I714891(E-P)
- Gel run for the digested samples.
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- Gel results:
- B7 - both screened colonies were negative (~3200bp of vector and ~3200bp of insert).
- B8 - only B8-5 shows the expected length for ligated plasmid (~3200bp of vector and ~4200bp of insert), but it also shows a single unexpected band...All the other 4 samples were negative (~3200bp of vector and ~3200bp of insert).
- A14-3 - showed the expected length for ligated plasmid (2079bp of vector and ~2200bp of insert).
- A16-4 - showed the expected length for ligated plasmid (2079bp of vector and ~1800bp of insert).
- I714891 - showed the expected length for ligated plasmid (~2700bp of vector and ~700bp of insert).
August, 25th
August, 26th
August, 27th
August, 28th
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Previous Week
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Next Week
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