"
Template:Team:KULeuven/29 August 2009/VanillinProduction
From 2009.igem.org
(Difference between revisions)
JochemDeen (Talk | contribs) (New page: * Split the restriction digest in 2 parts ** 1 Part was directly used for ligation using double the mass required {| border ="1" align="center" !| Part || DNA ng || volume µl |- align="ce...) |
JochemDeen (Talk | contribs) |
||
| Line 13: | Line 13: | ||
|- | |- | ||
|} | |} | ||
| - | * | + | * The 2nd part was first put on gel electrophorese and purified before it was used for ligation |
Revision as of 08:23, 31 August 2009
- Split the restriction digest in 2 parts
- 1 Part was directly used for ligation using double the mass required
| Part | DNA ng | volume µl |
|---|---|---|
| Sam5 | 100 | 0,8 |
| Sam8 | 126,4 | 2,7 |
| Ech | 100 | 1 |
| Fcs | 244 | 2,2 |
- The 2nd part was first put on gel electrophorese and purified before it was used for ligation
