Template:Team:KULeuven/29 August 2009/VanillinProduction

From 2009.igem.org

(Difference between revisions)
Line 20: Line 20:
***ech showed 4 results in gel (all too short), expected one
***ech showed 4 results in gel (all too short), expected one
** Sam5 and Sam8 were purified and ligated (Sams I)
** Sam5 and Sam8 were purified and ligated (Sams I)
 +
{| border ="1" align="center"
 +
!| Part || DNA conc ng/µl|| 260/280
 +
|- align="center"
 +
| Sam5||6,6 || 1,98
 +
|- align="center"
 +
| Sam8||4,1 || 2,88
 +
|- align="center
 +
|-}
* Ech and Fcs were cut again and put overnight (overweekend...) in 16C
* Ech and Fcs were cut again and put overnight (overweekend...) in 16C

Revision as of 09:59, 31 August 2009

  • Split the restriction digest in 2 parts
    • 1 Part was directly used for ligation using double the mass required(Sams II and Ef II)
Part DNA ng volume µl
Sam5100 0,8
Sam8126,4 2,7
Ech 100 1
Fcs 244 2,2
  • The 2nd part was first put on gel electrophorese and purified before it was used for ligation
  • Gel electrophorese of restriction on Sam, Sam8, fcs and ech
    • Sam5 and Sam8 showed expected results
    • fcs and ech was unexpected
      • fcs showed only 1 result on gel, expected two
      • ech showed 4 results in gel (all too short), expected one
    • Sam5 and Sam8 were purified and ligated (Sams I)
  • Ech and Fcs were cut again and put overnight (overweekend...) in 16C
Part DNA conc ng/µl 260/280
Sam56,6 1,98
Sam84,1 2,88
Retrieved from "http://2009.igem.org/Template:Team:KULeuven/29_August_2009/VanillinProduction"