Template:Team:KULeuven/12 August 2009/BlueLightReceptor

From 2009.igem.org

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#* Annealing of 58C
#* Annealing of 58C
# Innoculate liquid medium
# Innoculate liquid medium
-
#* Innoculation of sb3k3-{{kulpart|BBa_P1010}} and {{kulpart|BBa_J23101}}
+
#* Innoculation of {{kulpart|pSB3k3}}-{{kulpart|BBa_P1010}} and {{kulpart|BBa_J23101}}
# Ligation  
# Ligation  
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{| border ="1" align="center"

Revision as of 10:02, 1 September 2009

  1. Gel electrophorese of BlP cut with EcoRI and SpeI
  2. Gel extraction of the Blp

Nanodrop results

Part concentration (ng/μl) 260/280 λ 260/230 λ
Blp 27 2,08
  1. PCR (with Pfx) of blue light promoter with primers iGEM 2260 and igem 2261
    • Annealing of 58C
  2. Innoculate liquid medium
    • Innoculation of - and
  3. Ligation
Vector insert
+ (BLP)
50ng -> 2,5 μl 5 ng -> 0,3 μl
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