Template:Team:KULeuven/11 August 2009/BlueLightReceptor

From 2009.igem.org

(Difference between revisions)
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#* The cut should be 109 bp
#* The cut should be 109 bp
# Restriction digest
# Restriction digest
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# Electroporation of parts {{kulpart|psB3k3}}-{{kulpart|BBa_P1010}} and {{kulpart|BBa_J23101}} in electrocompetent cells
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# Electroporation of parts {{kulpart|pSB3K3}}-{{kulpart|BBa_P1010}} and {{kulpart|BBa_J23101}} in electrocompetent cells

Revision as of 12:07, 1 September 2009

  1. Restriction digest
    • cut with EcoRI and SpeI, incubation for 1,5 hour
  2. Gel electrophorese of
    • The cut should be 109 bp
  3. Restriction digest
  4. Electroporation of parts - and in electrocompetent cells