"
Team:Wash U/Calendar-Home/1 September 2009
From 2009.igem.org
(Difference between revisions)
(New page: 9/1 Minipreps {| |number |notation |concentration |- |A |RBS30_cph8 |168.0 |- |B |ompR_term_ompC_GFP |161.0 |- |C |ompR_term_ompC_pucB/A |188.0 |}) |
|||
| Line 18: | Line 18: | ||
|188.0 | |188.0 | ||
|} | |} | ||
| + | |||
| + | Did 2 digests of RBS30_cph8 with 1.5 ug each according to prefix-insertion instructions from the Silver lab- cut with EcoRI and PstI | ||
| + | Did 1 digest each of 1.5 ug of ompR_term_ompC_GFP and ompR_term_ompC_pucB/A as the vector for prefix insertion- cut with EcoRI and XbaI. | ||
| + | |||
| + | The Results of this digest are: | ||
| + | |||
| + | [[Image:9_1 gel purify.jpg]] | ||
Revision as of 20:27, 1 September 2009
9/1 Minipreps
| number | notation | concentration |
| A | RBS30_cph8 | 168.0 |
| B | ompR_term_ompC_GFP | 161.0 |
| C | ompR_term_ompC_pucB/A | 188.0 |
Did 2 digests of RBS30_cph8 with 1.5 ug each according to prefix-insertion instructions from the Silver lab- cut with EcoRI and PstI Did 1 digest each of 1.5 ug of ompR_term_ompC_GFP and ompR_term_ompC_pucB/A as the vector for prefix insertion- cut with EcoRI and XbaI.
The Results of this digest are:
